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Structure, Function and Expression Regulation of Hydrocarbon-degrading Enzymes and Their Encoding Genes(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2012 06
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Structure, Function and Expression Regulation of Hydrocarbon-degrading Enzymes and Their Encoding Genes
YU Hanying YANG Hui
(Enhanced Oil and Gas Recovery Key Laboratory of Ministry of Education, Department of Petroleum Engineering, Northeast Petroleum University, Daqing 163318, Heilongjiang, China)
petroleum microorganism hydrocarbon oxydase benzoylsuccinate sythase ethylbenzene dehydrogenase gene regulation
Q939.97 : Q78

The research on the enzymes and genes involving in hydrocarbon biodegradation is the important basis of molecular assay of petroleum microbes and construction of the engineering strains. In this review, the recent advances of structures, functions and regulations of hydrocarbon-degrading enzymes and the corresponding genes were summarized. The first enzymes in the aerobic metabolism pathways of hydrocarbon included several types of oxydases. Among them, the crystal structures of particulate methane monooxygenase protomer, the terminal oxygenase component of cumene dioxygenase and naphthalene dioxygenase were reported. The aryl-succinate or alkyl-succinate synthase were the first basic enzymes in the anaerobic transformation of hydrocarbon while ethylbenzene dehydrogenase catalyzed the initial reaction of anaerobic degradation of ethylbenzene in Azoarcus sp. For bacteria, the hydrocarbon-degrading genes consisting of operons and their transcriptions were induced by hydrocarbon or analogues and limited by global cell control. Some microorganisms possessed the complicated hydrocarbon-degrading regulations which were consistent with their various hydrocarbon metabolism pathways. In addition, the related study on ecology indicated that the induction process of genes involving in the hydrocarbon degradation in the environment were different from that of the culture in the laboratory. Based on the analysis of the unsolved questions of construction the hydrocarbon-degrading engineering bacteria, the significance of research on the comprehensive regulation of hydrocarbon metabolism in the cells and the induction of the related enzymes and genes in the environment was proposed. The application of the enzymes and genes, involving in hydrocarbon biodegradation, for the theoretic research on toxicant degradation and bioremediation in the future was prospected. Fig 3, Ref 50


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Last Update: 2012-12-28