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Microbial Characteristics and Influence Factors During Anaerobic Fermentation for Biohydrogen Production from CO(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2012 04
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Microbial Characteristics and Influence Factors During Anaerobic Fermentation for Biohydrogen Production from CO
ZHAO YaLIU ZhijunLIU FengxiaGUIOT Serge R
(1Faculty of Chemical & Environmental & Biological Science and Technology, Dalian University of Technology, Dalian 116024, China)
(2Biotechnology Research Institute, National Research Council of Canada, Montreal H4P 2R2, Canada)
biohydrogen production anaerobic fermentation thermophilic bacterium CO substrate inhibition
TK91 : TQ920.1

The biohydrogen production from anaerobic fermentation was studied with thermophilic bacterium Carboxydothermus hydrogenoformans using carbon monoxide (CO) dissolved in fermentation broth as the substrate. Bacterium growth model, decay efficient and the maximum specific growth rate were observed based on the experimental study of biomass growing, flocculation and microbial characteristics. The results indicated the feasibility of continuous anaerobic fermentation for hydrogen production from CO with C. hydrogenoformans due to its good hydrogen yield and flocculation ability. Furthermore, by investigating the effect of procedure parameters during CO fermentation, the optimal feed/microorganism was proposed and the maximum CO concentration was observed to avoid CO inhibition which was the key factor to consider in the study of continuous biohydrogen production from CO fermentation. Fig 4, Tab 2, Ref 17


Azbar N, Dokgoz FTC. The effect of dilution and l-malic acid addition on bio-hydrogen production with Rhodopseudomonas palustris from effluent of an acidogenic anaerobic reactor. Inter J Hydrogen Energy, 2010, 35 (10): 5028~5033
Wang YZ, Liao Q, Zhu X, Tian X, Zhang C. Characteristics of hydrogen production and substrate consumption of Rhodopseudomonas palustris CQK 01 in an immobilized-cell photobioreactor. Bioresour Technol, 2010, 101 (11): 4034~4041
XU LY (许丽英), Ren NQ (任南琪), Wang XZ (王兴祖), LI YF (李永峰), Zhang Y (张颖), XU H (徐慧), Chen GX (陈冠雄). Nitrogen nutrient and metabolic characteristics of hydrogen-producing novel species-Ethanoligenes harbinese B49. Chin J Appl Environ Biol (应用与环境生物学报), 007, 13 (2): 265~269
Wang JL, Wei W. Factors influencing fermentative hydrogen production: A review. Inter J Hydrogen Energy, 2009, 34 (2): 799~811
Chen GY (陈冠益), Gao WX (高文学), Ma WC (马文超). Review on hydrogen-rich gas production from biomass. Acta Energiae Solaris Sin (太阳能学报), 2006, 27 (12): 1276~1284
Zhang JC (张继春), Huang B (黄兵), Cao DF (曹东福). Progress on biohydrogen production by anaerobic fermentation. Yunnan Chem Technol (云南化工), 2007, 34 (2): 66~70
Wang YZ (王永忠), Liao Q (廖强), Zhu X (朱恂), Tian X (田鑫), Wang ZK (王中康), Zhang P (张攀), Zhang BP (张宝鹏). Isolation and identification of photosynthetic bacteria and their hydrogen-producing abilities. Chin J Appl Environ Biol (应用与环境生物学报), 2008, 14 (5): 673~677
Henstra AMStams AJM. Novel physiological features of Carboxydothermus hydrogenoformans and Thermoterrabacterium ferrireducens. Appl Environ Microbiol, 2004, 70 (12): 7236~7240
Svetlitchnyi V, Dobbek H, Meyer-Klaucke W, Meins T, Thiele B, Römer P, Huber R, Meyer O. A functional Ni-Ni-[4Fe-4S] cluster in the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans. Proc Natl Acad Sci USA, 2004, 101 (2): 446~451
Zhao Y, Cimpoia R, Liu ZJ, Guiot S. Kinetics of CO conversion into H2 by Carboxydothermus hydrogenoformans. Appl Microbiol & Biotechnol, 2011, 91 (6): 1677~1684
Zhao Y, Cimpoia R, Liu ZJ, Guiot S. Orthogonal optimization of Carboxydothermus hydrogenoformans culture medium for hydrogen production from carbon monoxide by biological water-gas shift reaction. Inter J Hydrogen Energy, 2011, 36 (17): 10655~10665
Qin Z (秦智), Ren NQ (任南琪), Li JZ (李建政). Bioaugmentation of hydrogen producing bacteria on operation of biohydrogen producing reactor. Environ Sci (环境科学), 2007, 28 (12): 2843~2848
Heijnen JJ. Bioenergetics of Microbial Growth. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. New York: John Wiley & Sons, 1999
Guiot S, Podruzny MF, McLean DD. Assessment of macroenergetic parameters for an anaerobic upflow biomass bed and filter (UBF). Biotechnol Bioeng, 1989, 34 (10): 1277~1288
Henstra AM. CO metabolism of Carboxydothermus hydrogenoformans and Archaeoglobus fulgidus: [Doctor Degree Dissertation]. Wageningen University, 2006
Soboh B, Linder D, Hedderich R. Purification and catalytic properties of a CO-oxidizing: H2-evolving enzyme complex from Carboxydothermus hydrogenoformans. Eur J Biochem, 2002, 269 (22): 5712~5721
17 Hursta KM, Lewis RS. Carbon monoxide partial pressure effects on the metabolic process of syngas fermentation. Biochem Eng J, 2010, 48 (2): 159~16


Last Update: 2012-08-21