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Advance in Research on Bacterial Aromatic Extradiol Dioxygenase(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2012 05
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Advance in Research on Bacterial Aromatic Extradiol Dioxygenase
XU Bingwen LI Shiyang ZHANG Qiang QU Yuanyuan MA Qiao LI Xinliang ZHOU Jiti ZHANG Xuwang ZHOU Hao
(Key Laboratory of Industrial Ecology and Environmental Engineering of Ministry of Eduction, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China)
extradiol dioxygenase classification catalytic mechanism biodegradation biosynthesis biotechnology
Q544 : Q939.97

As a type of bacterial aromatic ring-cleavage enzymes with versatile functions, extradiol dioxygenases (EDOs) have broad application in the field of environmental protection, chemical synthesis and biotechnologies. This paper reviewed the achievements of EDOs researches including the studies on their classification, catalytic mechanism, the application in biodegradation, biosynthesis, biotechnologies and the novel methods for extracting and modify EDOs. EDOs belong to three evolutionarily independent families, which utilize Fe(II) or Mn(II) as metal ions to catalyze the oxygenolytic fission of aromatic compounds via iron-alkylperoxo intermediates by the incorporation of substrates and O2. Through this mechanism, EDOs possess broad substrates spectra, and often take part in the biosynthesis of active compounds. Meanwhile, EDOs play an important role in the biotechnology such as biosensors. Recently, combined with the technology of metagenomic, hybrid enzymes, more and more EDOs were extracted and modified to provide a general message for the in depth study of EDOs. Fig 5, Ref 32


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Last Update: 2012-10-26