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Differences in Ecological Fitness Between Bt Transgenic Rice and Regular Rice Under Different Insect-infestation Pressures(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2012 01
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Differences in Ecological Fitness Between Bt Transgenic Rice and Regular Rice Under Different Insect-infestation Pressures
ZHANG Fuli LIU Yong TONG Hongjin YIN Quan TAO Li WANG Dong ZHOU Xiquan CHANG Lijuan SONG Jun LIU Wenjuan LEI Shaorong GUO Ling’an
(1Analysis and Determination Center, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China)
(2Plant Protection Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China)
(3Sichuan Academy of Environmental Protection Science, Chengdu 610064, China)
ecological fitness insect-resistance transgenic rice insect-infestation pressure gene spread ecological risk
Q788 : S511.034
DOI: 10.3724/SP.J.1145.2012.00035

For understanding the possibility and efficiency of foreign gene spread in nature and some potential ecological risk of transgenic rice, a experiment was carried out to investigate the effect of foreign Bt gene insertion on the ecological fitness of the transgenic rice plant. Three Bt transgenic rice cultivars, ‘Bt63’, ‘R1’ and ‘R2’, and a non-transgenic rice ‘Ⅱ-838’ were used as materials. The insect-resistant transgenic and the non-transgenic rice plants were inter-cropped pair-wisely. Two levels of rice stem borer-infestation were set up. The vegetative growth, seed-setting and the resistance to rice stem borers were compared between transgenic and non-transgenic lines. The results showed that both number of tillers and fresh weight of the transgenic rice plant were no differences compared with the control under low pest-infestation pressure, but the plant height, spike length and weight were all lower than those of the non-transgenic rice plant, and ‘Bt63’ and ‘R2’ were significantly different compared with the control. On the contrary, the number of tillers, spike weight and the length of the transgenic rice plant under high pest-infestation level were significantly higher than those of the control. The plant height was different amongst the transgenic lines, which was presumably because of the receptive cultivars’ traits. Both the number of seeds and thousand-grain weight were no differences between the transgenic and non-transgenic rice lines under two different insect-infestation pressures, suggesting that the effect of foreign Bt gene on seed setting was not significant. In contrast to the non-transgenic rice, three Bt transgenic rice lines displayed a higher resistance to rice stem borer. The fitness of the Bt transgenic rice was distinctly higher under high pest-infestation pressure, which indicated that the effect of foreign Bt gene on the insect-resistance of plant recipients was distinct. At the same time, lower fitness cost of Bt transgenic rice in this experiment implied that it was possible that foreign Bt gene would flow away under certain conditions, but this risk was very low. Fig 3, Tab 2, Ref 27


1 Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol & Mol Biol Rev, 1998, 62 (3): 775~806
2 Lin LB (林良斌),Guan CY (官春云). Bt toxic protein gene and gene engineering of insect-resistant plant. Progr Biotechnol (生物工程进展), 1997, 17 (2): 51~55
3 Wang HY (汪海燕). Study on environmental behavior and bioeffects of bt transgenic rice and expressed products of its exogenous gene: [Doctor Degree Dissertation]. Hangzhou, China: Zhejiang University (杭州: 浙江大学), 2007
4 Shu QY, Cui HR, Ye GY, Wu DX, Xia YW, Gao MW, Altosaar I. Agronomic and morphological characterization of Agrobacterium-transdormed Bt rice plants. Euphytica, 2002, 127: 345~352
5 Ye GY, Yao HW, Shu QY, Cheng X, Hu C, Xia YW, Gao MW, Altosaar I. High levels of stable resistance in transgenic rice with a crylAb gene from bacillus thuringiensis Berliner to rice leaf folder, Cnaphalocrocis medinalis (Guenee) under field conditions. Crop Prot, 2003, 22: 171~178
6 Shu QY, Ye GY, Cui HR, Cheng XY, Xiang YB, Wu DX, Gao MW, Xia YW, Hu C, Sardana R, Altosaar I. Transgenic rice plants with a synthetic crylAb gene from Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species. Mol Breeding, 2000, 6: 433~439
7 Tu JM, Zhang GA, Datta K, Xu CG, He YQ, Zhang QF, Khush GS, Datta SK. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin. Nat Biotechnol, 2000, 18:1101~1104
8 Zeng QC (曾千春), Wu Q (吴茜), Zhou KD (周开达), Feng DJ (冯德江), Wang F (王锋), Shu J (苏军), Altosaar I, Zhu Z (朱祯). Obtaining stem borer resistant homozygous transgenic lines of Minghui 81 harboring novel cry1ac gene via particle bombardment. Acta Genet Sin (遗传学报), 2002, 29 (6): 519~524
9 Liu YF (刘雨芳). Advance on transgenic Bt rice and ecology safety evaluation. Life Sci Res (生命科学研究), 2004, 8 (4): 294~299
10 Tang L (唐丽), Tan YN (谭炎宁), Han XX (韩小霞), Sun ZZ (孙志忠), Duan MJ (段美娟). Research progress and developing tendency in insect-resistant transgenic rice. Hybrid Rice (杂交水稻), 2011, 26 (1): 1~6
11 Burke JM, Rieseberg LH. Fitness effects of transgenic disease resistance in sunflowers. Science, 2003, 300 (5623): 1250
12 Chen LY, Snow AA, Wang F, Lu BR. Effect of insect-resistance transgenes on fecundity in rice (Oryza sativa, Poaceae): A test for underlying costs. AmJ Bot, 2006, 93 (1): 94~101
13 Clark EA. Environmental risks of genetic engineering. Euphytica, 2006, 148: 47~60
14 Wang Z (王舟). Risk assessment of gene flow from herbicide-resistant genetically modified rice and oil seed rape respectively to weedy rice and wild brown mustard under artificial cultural conditions through ecological fitness: [Master Degree Dissertation]. Nanjing, China: Nanjing Agricultural University (南京: 南京农业大学), 2007
15 Lu BR (卢宝荣), Zhang WJ (张文驹), Li B (李博). Escape of transgenes and its ecological risks. Chin J Appl Ecol (应用生态学报), 2003, 14 (6): 989~995
16 Song ZP, Li B, Chen JK, Lu BR. Genetic diversity and conservation of common wild rice (Oryza rufipogon) in China. Plant Species Biol, 2005, 20 (2): 83~92
17 Lu BR, Snow AA. Gene flow from genetically modified rice and its environmental consequences. Bioscience, 2005, 55 (8): 669~678
18 Bolette PP, Paul N, Christian A, Stephen BP. Ecological fitness of a glyphosate-resistant Lolium rigidum population: Growth and seed production along a competition gradient. Basic & Appl Ecol, 2007, 8: 258~268
19 Lu BR (卢宝荣), Fu Q (傅强), Shen ZC (沈志成). Commercialization of transgenic rice in China: Potential environmental biosafety issues. Biodiv Sci (生物多样性), 2008, 16 (5): 426~436
20 Lu BR, Yang C. Gene flow from genetically modified rice to its wild relatives: Assessing potential ecological consequences. Biotechnol Adv, 2009, 27 (6): 1083~1091
21 Cao QJ, Xia H, Yang X, Lu BR. Performance of hybrids between weedy rice and insect-resistant transgenic rice under field experiments: implication for environmental biosafety assessment. J Integr Plant Biol, 2009, 51 (12): 1138~1148
22 GB/T 5519-2008. Cereals and pulses – Determination of the mass of 1000 grain. Beijing, China: Standards Press of China (北京: 中国标准出版社), 2008
23 Rieseberg LH, Burke JM. The biological reality of species: Gene flow, selection, and collective evolution. Taxon, 2001, 50 (1): 47~67
24 Chen LY (陈良燕). Fitness effect of insect-resistance transgenes on rice act as receptors: [Doctor Degree Dissertation]: Shanghai, China: Fudan University (上海: 复旦大学), 2005
25 Zhang L (张磊), Zhu Z (朱祯). Effect of transgenic insect-resistant rice on biodiversity. Hereditas (遗传), 2011, 33: 1~8
26 Snow AA, Pilson D, Riseberg LH, Paulsen MJ, Pleskac N, Reagon MR, Wolf DE, Selbo SM. A Bt transgene reduces herbivory and enhances fecundity in wild sunflowers. Ecol Appl, 2003, 13 (2): 279~286
27 Chen LJ, Lee DS, Song ZP, Suh HS, Lu BR. Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Ann Bot, 2004, 93: 67~73


Last Update: 2012-02-29