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Enhancing Expression of ywtD Gene in Bacillus licheniformis WX-02 by Two Types of Promoters(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2012 03
Page:
450-454
Research Field:
Articles
Publishing date:

Info

Title:
Enhancing Expression of ywtD Gene in Bacillus licheniformis WX-02 by Two Types of Promoters
Author(s):
FU Juntao QI Gaofu LIU Jun JI Zhixia MA Xin CHEN Shouwen
(1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China)
(2College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China)
Keywords:
poly-γ-glutamate B. licheniformis depolymerase gene SacB promoter α-amylase gene promoter enhancing expression
CLC:
Q936 : Q786
PACS:
DOI:
10.3724/SP.J.1145.2012.00450
DocumentCode:

Abstract:
Poly-γ-glutamate (γ-PGA) is a biopolymer material that has a good application prospect. The effect of two promoters on the ywtD gene expression in Bacillus licheniformis WX-02 was investigated in this study to obtain highly expressed γ-PGA depolymerase YwtD. Two recombinants of B. licheniformis WX-02, SYT and PYT, in which ywtD gene was expressed from the control of SacB promoter and α-amylase gene promoter, respectively, were constructed. The enzyme activity assay showed that the expression of YwtD proteins were enhanced in the recombinants SYT and PYT. Both B. licheniformis recombinants SYT and PYT could reduce the molecular weight of γ-PGA from 1 000 000~1 200 000 to 800 000~900 000. In addition, PYT resulted in a 33% increase of γ-PGA production from 13.50 to 17.97 g L-1, whereas γ-PGA production of SYT reduced to 10.85 g L-1. Therefore, the promoter of α-amylase gene was more suitable for constructing the engineering strain with a higher yield and reduced molecular weight of γ-PGA.

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Last Update: 2012-06-19