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Isolation, Identification and Phylogenetic Analysis of an Alkalophilic Methanosarcina(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2012 02
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Isolation, Identification and Phylogenetic Analysis of an Alkalophilic Methanosarcina
QIAN Dandan MA Longlong YUAN Yuexiang YAN Zhiying LIU Xiaofeng LIAO Yinzhang HE Rongna
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Graduate University of Chinese Academic of Sciences, Beijing 100049, China)
(3Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China)
alkalophilic methanogen strain isolation and identification anaerobic fermentation phylogenetic analysis 16S rDNA

An alkalophilic methanogen T13 was isolated by anaerobic techniques of Hungate from cow muck in Hongyuan county, Sichuan, China enriched in culture for methanogen at 15 ℃. Its optimum pH for producing methane was 8.5, and the temperature for growth was observed at the range of 8~45 ℃. The culture was visible particulate. Cells were aggregated and gram-positive. The colony was pale yellow, mulberry-like and fluoresced. T13 could grow using acetate, methanol or methylamine as sole carbon source, and it was sensitive to chloramphenicol and gentamicin. The optimum temperature and NaCl concentration for its growth were 30 ℃ and 0~0.15 mol L-1, respectively. It grew at the pH range between 6.5 and 9.5. The morphological and physio-biochemical characteristics combined with 16S rDNA sequence analysis of strain T13 demonstrated that it was a strain of Methanosarcina mazei. Since its optimum pH for growth was 8.5, strain T13 therefore was considered preliminarily as a new strain of M. mazei. Fig 5, Tab 1, Ref 18


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Last Update: 2012-04-27