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Diversity of Virus-like Particles from High-temperature Acidic Hot Springs in Tengchong Rehai, China(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2012 02
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Diversity of Virus-like Particles from High-temperature Acidic Hot Springs in Tengchong Rehai, China
DANG Yafeng CHEN Bo ZHANG Qi HUANG Xiaoxing WEI Yunlin LIN Lianbing
(1Biotechnology Research Center, Kunming University of Science and Technology, Kunming 650500, Yunnan, China)
(2State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of sciences, Beijing 100101, China)
Tengchong Rehai virus diversity extreme environment Archaea STSV2 (Sulfolobus tengchongensis spindle-shaped virus 2)

For exploring and characterizing the diversity of virus particles from high-temperature acidic hot springs in Tengchong Rehai in Yunnan Province, China, by using electronic microscopy, double-layer plate and DNA restriction analysis, virus particles were identified from enriched water samples of acidic hot springs with temperature between 61~94 ℃ in Tengchong Rehai, and their morphology was further characterized. Four types of virus-like particles, head and tail-shaped virus, linear virus, spherical virus and spindle-shaped virus, were observed in Tengchong Rehai hot springs by their morphological characteristics. These particles were morphologically similar to those isolated from the same environments in the United States, Japan, Iceland, and some other places and most of them were Sulfolobus virus. A Sulfolobus bacterium virus with an average length of about 50 nm was subsequently isolated, having a spindle head of 220 nm× 80 nm, a tail 20 to 100 nm in length at one end and an envelope about 10~15 nm in thickness. According to its morphological characteristics significantly different from that of Sulfolobus virus STSV1, this virus was proposed to be a new Sulfolobus virus and designated as STSV2 (Sulfolobus tengchongensis spindle-shaped virus 2). Fig 7, Ref 21


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Last Update: 2012-04-27