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Horizontal Gene Transfer Between Sclerotinia sclerotiorum and Bacteria(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2012 01
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Horizontal Gene Transfer Between Sclerotinia sclerotiorum and Bacteria
LÜ Linfeng YUE Jianyu FEI Zhong’an ZHAO Rui QIAO Dairong CAO Yi
(Sichuan Public Experimental Platform of Bioinformatics and Metabolic Engineering, College of Life Sciences, Sichuan University, Chengdu 610064)
horizontal gene transfer (HGT) Sclerotinia sclerotiorum phylogenetic tree GC-content
S435.654 : Q933

Sclerotinia sclerotiorum is one of the most terrible diseases for rapeseeds in production. Its pathogenicity may probably get from horizontal gene transfer (HGT). For understanding its pathogenic mechanism and finding targets for new fungal inhibitors, firstly in BLASTp comparison results of gene XM_001585458.1-coded proteins XP_001585508.1 of different species, low E value 3.23e-109 and high SCORE value 436 appeared in the results of bacteria, showing HGT happened probably. Then via phylogenetic method, gene XM_001585458.1 was found out the closest to bacterium. At the same time, by nucleotide composition analysis, XM_001585458.1 also appeared quite different from the genome of S. sclerotiorum, since GC-content increased 14.95%. To sum up, HGT of gene XM_001585458.1 was found out. And structure and COG analysis indicated that there were two distinct transmembrane domains in 14~171 and 465~485 of protein XP_001585508.1, and this protein also consisted of 6 β-sheets, 1 α-helix, some turns, and random coils. So the candidate protein encoded by the transferring gene should be further predicted to be a membrane protein participating in polysaccharide metabolic. And it can be assumed that the gene transferring is related to the pathogenicity of S. sclerotiorum and its activity of hydrolyzing the cell wall when it is infecting the plant. Fig 3, Tab 3, Ref 17


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Last Update: 2012-02-29