|Table of Contents|

Optimization of Growth Conditions of in Vitro Shoots of Walnut ‘Xiangling’(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2011 03
Research Field:
Publishing date:


Optimization of Growth Conditions of in Vitro Shoots of Walnut ‘Xiangling’
JI Aiqing ZHU Chao WU Guoliang
(1Faculty of Horticulture, Shanxi Agricultural University, Taigu 030801, Shanxi, China)
(2Faculty of Biology Science and Technology, Jinzhong University, Jinzhong 030600, Shanxi, China)
(3Faculty of Horticulture, Henan Agricultural University, Zhengzhou 450002, China)
walnut ‘Xiangling’ in vitro shoot basic medium gelling agent cytokinin proliferation elongation
S664.104.3 : Q943.1

To provide abundant and excellent colonial plant materials, the optimum growth conditions of in vitro walnut shoot were studied. Using the seedlings of walnut ‘Xiangling’ as materials, the types of nutrient media (MS, DKW and WPM) and gelling agents (Difco Bacto agar, Phytagel and a mixture of Phytagel and Difco Bacto agar) were screened, and the effects of different types and concentrations of cytokinin on growth of the seedlings were studied with randomized complete-block design. The results indicated that different types of nutrient media and gelling agents had great effects on growth of the seedlings as well as the types and concentrations of cytokinin. DKW basic medium supplemented with 0.8 mg/L BA + 0.01 mg/L IBA and solidified with 2.2 g/L Phytagel, favoured proliferation of the shoots, and the number of proliferation was 4.70. After cultured in present of 0.8 mg/L KT + 0.01 mg/L IBA for strong stock, the shoots could be rooted. Different genotypes need basic media with different compositions of inorganic salt. Phytagel containing rich minerals is helpful for bud and shoot growth. BA promotes axillary to germinate and proliferate, while KT is helpful for shoot elongation. Fig 4, Tab 3, Ref 22


1 Wu GL (吴国良). 核桃无公害高效生产技术. Beijing, China: Chinese Agriculture Press (北京: 中国农业出版社), 2010
2 Gao QH (高清华), Duan K (段可), Gan L (甘霖), Zhang DF (张大福). Advances in walnut propagation gechniques in China. J Fruit Sci (果树学报), 2000, 17 (3): 220~224
3 Tian AM (田爱梅). Study on factors affecting tissue culture of axillary shoots of seedling walnut: [Master’s Digree Thesis]. Taigu, China: Shanxi Agriculture University (太谷: 山西农业大学), 2001
4 Ji AQ (冀爱青). Study of factors affecting on somatic embryogenesis in walnut. [Master’s Digree Thesis]. Taigu, China: Shanxi Agriculture University (太谷: 山西农业大学), 2003
5 Zhang Y (张燕). Study on the problems in the tissue culture of Fenyang walnut. J Anhui Agric Sci (安徽农业科学), 2006, 34 (11): 2365~2367
6 Liu LY (刘兰英).Tissue culture and rapid propagation of Juglans regia L. Plant Physiol Commun (植物生理通讯), 2000, 36 (5): 434~435
7 Tang HR (汤浩茹), Wang YQ (王永清), Ren ZL (任正隆), Krczal G.. Somatic embryogenesis and plant regeneration from embryonic axes and cotyledons of walnut immature embryos of cv. No.120. Acta Hortic Sin (园艺学报), 2000, 27 (1): 59~61
8 Fang HJ (方宏筠), Wang GL (王关林). Somatic embryogenesis of Juglans nigia L. and establishment of gene transformation system of walnut. Acta Hortic Sin (园艺学报), 2000, 27 (6): 406~411
9 Xing RD (邢瑞丹), Li YD (李亚东), Liu QZ (刘庆忠), Li GT (李国田), Chen X (陈新), Chen M (陈明). Adventitious shoot regeneration from in vitro leaf of Juglans regia cv. Xiangling. J Fruit Sci (果树学报), 2010, 27 (1): 146~149
10 Zhang JL (张俊林), Liu QZ (刘庆忠), Fan J (樊靖), Qin L (秦岭). 核桃茎尖增殖培养基筛选试验. China Fruits (中国果树), 2008 (2): 40~42
11 Ponehia G, Tonon G. Preliminary result of research on micropropagation of walnut. Rivista di Frutticoltura, 1993, 55 (l): 91~94
12 Zhu YQ (朱玉球), Liao WY (廖望仪), Huang JQ (黄坚钦), Sun XP (孙晓萍). A preliminary study on induction of callus in Carya cathayensis. J Zhejiang For Coll (浙江林学院学报), 2001, 18 (2): 115~118
13 Li JJ (李建军), Li MJ (李明军). The primary study of Juglans nigra in vitro culture. J Henan Agric Sci (河南农业科学), 2005 (9): 64~66
14 George EF, Puttock DJM, George HJ. Plant Culture Media. Edington, US: Exegetics, 1987. 987
15 Liu SL (刘淑兰), Han BW (韩碧文). In vitro propagation of walnut (Juglans regia L.). Acta Agric Univ Pekinensis (北京农业大学学报), 1986, 12 (2): 143~147
16 Gruselle R, Boxus P. Walnut micropropagation. Acta Hort, 1990, 284: 45~52
17 Fatima A, Kamili AN, Shah AM. Plantlet regeneration from excised embryonal axes, shoot apices, and nodal segments of Juglans regia L. Acta Hort, 2006, 705: 387~392
18 Xie QX (谢启鑫), Huang ML (黄美连), Wu XP (吴晓萍), Zhuang DH (庄东红). Plant regeneration from leaves of date plum (Diospyros lotus L.). Scientia Agric Sin (中国农业科学), 2008, 41 (2): 607~612
19 Huetteman CA, Preece JE. Thidiazuron: A potent cytokinin for woody plant cell tissue culture. Plant Cell Tiss Org Cult, 1993, 33: 105~121
20 Pawlicki G, Welander M. Adventitious shoot regeneration from leaf segments of in vitro cultured shoots of the apple rootstock ‘Jork 9’. J Hort Sci, 1994, 69 (4): 687~696
21 Caboni E, Tonelli MG, Lauri P, Angeli SD.Damiano C. In vitro shoot regeneration from leaves of wild pear. Plant Cell Tiss Org Cult, 1999, 59: 1~7
22 Yu YJ (于亚军), Dai HP (代汉萍), Li BJ (李宝江). 植物激素和生长调节在果树组织培养中的应用. North Hort (北方园艺), 2002 (6): 68~70


Last Update: 2011-06-23