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Optimization of Growth Conditions of in Vitro Shoots of Walnut ‘Xiangling’(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2011 03
Page:
359-363
Research Field:
Articles
Publishing date:

Info

Title:
Optimization of Growth Conditions of in Vitro Shoots of Walnut ‘Xiangling’
Author(s):
JI Aiqing;  ZHU Chao;  WU Guoliang
(1Faculty of Horticulture, Shanxi Agricultural University, Taigu 030801, Shanxi, China)
(2Faculty of Biology Science and Technology, Jinzhong University, Jinzhong 030600, Shanxi, China)
(3Faculty of Horticulture, Henan Agricultural University, Zhengzhou 450002, China)
Keywords:
walnut ‘Xiangling’;  in vitro shoot;  basic medium;  gelling agent;  cytokinin;  proliferation;  elongation
CLC:
S664.104.3 : Q943.1
PACS:
DOI:
10.3724/SP.J.1145.2011.00359
DocumentCode:

Abstract:
To provide abundant and excellent colonial plant materials, the optimum growth conditions of in vitro walnut shoot were studied. Using the seedlings of walnut ‘Xiangling’ as materials, the types of nutrient media (MS, DKW and WPM) and gelling agents (Difco Bacto agar, Phytagel and a mixture of Phytagel and Difco Bacto agar) were screened, and the effects of different types and concentrations of cytokinin on growth of the seedlings were studied with randomized complete-block design. The results indicated that different types of nutrient media and gelling agents had great effects on growth of the seedlings as well as the types and concentrations of cytokinin. DKW basic medium supplemented with 0.8 mg/L BA + 0.01 mg/L IBA and solidified with 2.2 g/L Phytagel, favoured proliferation of the shoots, and the number of proliferation was 4.70. After cultured in present of 0.8 mg/L KT + 0.01 mg/L IBA for strong stock, the shoots could be rooted. Different genotypes need basic media with different compositions of inorganic salt. Phytagel containing rich minerals is helpful for bud and shoot growth. BA promotes axillary to germinate and proliferate, while KT is helpful for shoot elongation. Fig 4, Tab 3, Ref 22

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Last Update: 2011-06-23