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Differential Expression and Gene Cloning of Peroxiredoxin from Nostoc flagelliforme Subjected to Dehydration and Rehydration(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2011 05
Page:
662-672
Research Field:
Articles
Publishing date:

Info

Title:
Differential Expression and Gene Cloning of Peroxiredoxin from Nostoc flagelliforme Subjected to Dehydration and Rehydration
Author(s):
LIANG WenyuJIAO GuangfeiZHOU YouwenYOU XiangrongZHANG YapingCHEN Wei
(1School of Life Sciences, Ningxia University, Yinchuan 750021, China )
(2College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
(3Institute of Agro-food Science & Technology, Guangxi Academy of Agricultural Sciences, Nanning 530007 , China)
Keywords:
-
CLC:
Q945.78 : Q78
PACS:
DOI:
10.3724/SP.J.1145.2011.00666
DocumentCode:

Abstract:
Nostoc flagelliforme is a terrestrial nitrogen-fixing cyanobacteria with strong drought ecological adaptability. In this research, 2-DE, gel image analysis and MALDI-TOF-TOF/MS were applied to compare and identify the peroxiredoxin expression level from N. flagelliforme subjected to dehydration and rehydration. Degeneracy primer was designed based on the identified amino acid sequences in order to clone peroxiredoxin gene, to analyze the gene and amino acid sequences, to predict the secondary structure of peroxiredoxin, and to study the prokaryotic expression of the peroxiredoxin gene. The results indicated that peroxiredoxin expression level of N. flagelliforme subjected to rehydration was higher than that to desiccation. Peroxiredoxin gene was cloned and a full length of 639 bp DNA was obtained (GenBank access number: HM854286). Homology analysis showed that the N. flagelliforme peroxiredoxin gene has high consensus regions. The secondary structure of peroxiredoxin was made up of α helix, β strands and random coil. Peroxiredoxin gene was expressed in E. coli, and a Mr 26.5×103 heterologous protein was obtained. It was confirmed by Western blotting that the protein was peroxiredoxin. The study laid a foundation for in depth research in terms of the drought-resistant molecular mechanism and protection mechanism of N. flagelliforme in extreme arid environment. Fig 10, Tab 1, Ref 25

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Last Update: 2011-10-25