|Table of Contents|

Differential Expression and Gene Cloning of Peroxiredoxin from Nostoc flagelliforme Subjected to Dehydration and Rehydration(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2011 05
Research Field:
Publishing date:


Differential Expression and Gene Cloning of Peroxiredoxin from Nostoc flagelliforme Subjected to Dehydration and Rehydration
LIANG WenyuJIAO GuangfeiZHOU YouwenYOU XiangrongZHANG YapingCHEN Wei
(1School of Life Sciences, Ningxia University, Yinchuan 750021, China )
(2College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
(3Institute of Agro-food Science & Technology, Guangxi Academy of Agricultural Sciences, Nanning 530007 , China)
Q945.78 : Q78

Nostoc flagelliforme is a terrestrial nitrogen-fixing cyanobacteria with strong drought ecological adaptability. In this research, 2-DE, gel image analysis and MALDI-TOF-TOF/MS were applied to compare and identify the peroxiredoxin expression level from N. flagelliforme subjected to dehydration and rehydration. Degeneracy primer was designed based on the identified amino acid sequences in order to clone peroxiredoxin gene, to analyze the gene and amino acid sequences, to predict the secondary structure of peroxiredoxin, and to study the prokaryotic expression of the peroxiredoxin gene. The results indicated that peroxiredoxin expression level of N. flagelliforme subjected to rehydration was higher than that to desiccation. Peroxiredoxin gene was cloned and a full length of 639 bp DNA was obtained (GenBank access number: HM854286). Homology analysis showed that the N. flagelliforme peroxiredoxin gene has high consensus regions. The secondary structure of peroxiredoxin was made up of α helix, β strands and random coil. Peroxiredoxin gene was expressed in E. coli, and a Mr 26.5×103 heterologous protein was obtained. It was confirmed by Western blotting that the protein was peroxiredoxin. The study laid a foundation for in depth research in terms of the drought-resistant molecular mechanism and protection mechanism of N. flagelliforme in extreme arid environment. Fig 10, Tab 1, Ref 25


1 Kroll J. Molecular chaperones and the process of cellular immortalization in vitro. Bilgerontology, 2002, 3: 183~185
2 Yang L (杨柳), Mi C (米粲), Huo YY (霍艳英). Role of peroxiredoxin in H2O2 mediated signaling. Intern J Pathol & Clin Med (国际病理科学与临床杂志), 2006, 26 (5): 417~419
3 Mehdy MC. Active oxygen species in plant defense against pathogens. Plant Physiol, 1994, 105: 467~472
4 Gao KS. Chinese studies on the edible blue-green alga, Nostoc flagelliforme, a review. J Appl Phycol,1998, 10: 37~49
5 Cameron RE. Species of Nostoc vaucher occuring in the Sonoran Desert in Arizona. Tran Am Microsc Soc, 1962, 81: 379~384
6 Qian KX (钱凯先), Zhu HR (朱浩然), Chen SG (陈树谷). The ecological conditions for Nostoc flagelliforme and their analysis. Acta Phytoecol Geobot Sin (植物生态学与地植物学报), 1989, 13 (2): 97~105
7 Patterson BD, Macrae EA, Feaguson IB. Estimation of hydrogen peroxide in plant extracts using titanium (IV). Anal Biochem,1984, 139: 487~492
8 Angelini R, Manes F, Federico R. Spatial and functional correlation between diamine-oxidase and peroxidase activities and their dependence upon deetiolation and wounding in chick-pea stems. Planta, 1990, 182: 89~96
9 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 7: 248~254
10 Liang WY (梁文裕), Wang X (王星), Jiao GF (焦广飞), Zhang XJ (张喜娟), Chen W (陈伟). Establishment and optimization of two-dimensional gel electrophoresis for proteome of Nostoc flagelliforme. Acta Bot Bor.-Occid Sin (西北植物学报), 2009, 29 (8): 1550~1556
11 Liu H, Liu YZ, Zheng SQ, Jiang JM, Wang P, Chen W. Comparative proteomic analysis of longan (Dimocarpus longan Lour.) seed abortion. Planta, 2010, 231: 847~860
12 Duan BL (段宝利), Yin CY (尹春英), Li CY (李春阳). Responses of conifres to drought stress. Chin J Appl Environ Biol (应用与环境生物学报), 2005, 11 (1): 115~122
13 Yang F (扬帆), Miao LF (苗灵凤), Xu X (胥晓), Li CY (李春阳). Progress in research of plant responses to drought stress. Chin J Appl Environ Biol (应用与环境生物学报), 2007, 13 (4): 586~591
14 Shirkey B, Kovarcik DP, Wright DJ. Active Fe-containing superoxide dismute and abundant sodF mRNA in Nostoc commune (Cyanobacteria) after years of desiccation. J Bact, 2000, 182 (2): 189~197
15 Sedgwick SG, Smerdon SJ. The ankyrin repeats: A diversity of nteractions on common structural framework. Trends Biochem Sci, 1999, 24 (8): 311~316
16 Latifi A, Ruiz M, Jeanjean R, Zhang CC. PrxQ-A, a member of the peroxiredoxin Q family, plays a major role in defense against oxidative stress in the cyanobacterium Anabaena sp. strain PCC7120. Free Radical Biol & Med, 2007, 42: 424~431
17 Naomi HM, Ken M, Hidehisa Y, Akiko N, Kazuhito I, Masayuki O, Toru H. Anti-oxidative stress system in cyanobacteria. Biolog Chem, 2005, 280 (1): 840~846
18 Sueoka K, Yamazaki T, Hiyama T, Nakamoto H. The NADPH thioredoxin reductase C functions as an electron donor to 2-Cys peroxiredoxin in a thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Biochem & Biophys Res Commun, 2009, 380 (3): 520~524
19 Tang JN (唐进年), Zhao M (赵明), Zhang DM (张盹明), Zhang JC (张锦春), Nie WG (聂文果). Water physiological characteristics of Nostoc flagelliforme. Acta Bot Bor-Occid Sin (西北植物学报), 2005, 25 (2): 236~243
20 Zhao XM, Bi YH, Chen L, Hu S, Hu ZY. Responses of photosynthetic activity in the drought-tolerant cyanobacterium, Nostoc flagelliforme to rehydration at different temperature. J Arid Environ, 2008, 72: 370~377
21 Bi H (毕永红), Hu ZY (胡征宇). Effect of water on physiological activity of Nostoc flagelliforme. J Wuhan Bot Res (武汉植物学研究), 2003, 21 (6): 503~507
22 Wei LZ, Ma LM, Wang QX, Mi HL. Effect of cell amount on photosynthetic yield in the cyanobacterium Nostoc flagelliforme and involovement of NADPH dehydrogenase-mediated cyclic electron transport. J Appl Phycol, 2009, 21: 179~184
23 Xie X (解筱), Yang B (杨波), Chen YH (陈月辉). Prediction of secondary structure of protein using neural network. J Jinan Univ Sci & Technol (济南大学学报自然科学版), 2008, 22 (2): 111~115
24 Wang Y (汪莹), Chen LP (陈李萍), Chen X (陈晓), Zhang X (章秀), Yu J (于晶), Wang QX (王全喜). Cloning and expression of gene which encode SOD of Nostoc flagelliforme in E. coli. Bull Bot Res (植物研究), 2007, 27 (3): 289~292
25 Zhang X (章秀). Cloning, expression of gene encoding new trehalose-producing enzyme and trehalose-hydrolyzing enzyme from Nostoc flagelliforme; [Degreer Dissertation]. Shanghai, China: Shanghai Normal University (上海师范大学), 2008. 18~49


Last Update: 2011-10-25