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Cloning and Expression Analysis of Sucrose-phosphate Synthase II Gene from Sugarcane(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2011 05
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Cloning and Expression Analysis of Sucrose-phosphate Synthase II Gene from Sugarcane
YE BingyingQIU SiZHOU PingSONG XimeiCHEN YouqiangCHEN Rukai
(1College of Life Sciences, Fujian Normal University, 2Key Laboratory of Sugarcane Genetics and Breeding, Ministry of Agriculture, 3Fruit Research Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350108, China)
(4South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, Guangdong, China)
sucrose phosphate synthase gene cloning expression analysis real-time PCR sugarcane
Q78 : S566.101

To achieve the ultimate aim of increasing sucrose content in the sugarcane stem, it is necessary to investigate the principal rate-limiting steps in the pathway of sucrose synthesis. It has been suggested that sucrose phosphate synthase (SPS) is a key rate-limiting enzyme in sucrose formation and it also influences sucrose accumulation and carbon partitioning processes. Relative expression of SPSⅡ gene occupies 40% of the total transcription of SPS gene, which shows that the cloning and expression of SPSⅡ gene influences sucrose accumulation process. This research focused on molecular cloning and expression analysis of SPSⅡ gene in sugarcane. The SPSⅡ cDNA fragment was isolated from sugarcane, which contained a 3 183 bp open reading frame (ORF), encoding 1 060 amino acids. Its Genbank accession no. EU 269038. Relative expression of SPSⅡ gene was determined in the different tissues of sugarcane at different time of sugar accumulation by real-time PCR. The results showed highest relative expression of SPSⅡ gene in the canes at early stage during accumulation process of sucrose, but highest in the leaves at middle stage. In the same photosynthetic tissue, relative expression of SPSⅡ was higher at early stage during accumulation process of sucrose than those at middle stage and later stage. Fig 6, Ref 11


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Last Update: 2011-10-25