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Cloning and Expression Analysis of Sucrose-phosphate Synthase II Gene from Sugarcane(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2011 05
Page:
673-677
Research Field:
Articles
Publishing date:

Info

Title:
Cloning and Expression Analysis of Sucrose-phosphate Synthase II Gene from Sugarcane
Author(s):
YE BingyingQIU SiZHOU PingSONG XimeiCHEN YouqiangCHEN Rukai
(1College of Life Sciences, Fujian Normal University, 2Key Laboratory of Sugarcane Genetics and Breeding, Ministry of Agriculture, 3Fruit Research Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350108, China)
(4South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, Guangdong, China)
Keywords:
sucrose phosphate synthase gene cloning expression analysis real-time PCR sugarcane
CLC:
Q78 : S566.101
PACS:
DOI:
10.3724/SP.J.1145.2011.00673
DocumentCode:

Abstract:
To achieve the ultimate aim of increasing sucrose content in the sugarcane stem, it is necessary to investigate the principal rate-limiting steps in the pathway of sucrose synthesis. It has been suggested that sucrose phosphate synthase (SPS) is a key rate-limiting enzyme in sucrose formation and it also influences sucrose accumulation and carbon partitioning processes. Relative expression of SPSⅡ gene occupies 40% of the total transcription of SPS gene, which shows that the cloning and expression of SPSⅡ gene influences sucrose accumulation process. This research focused on molecular cloning and expression analysis of SPSⅡ gene in sugarcane. The SPSⅡ cDNA fragment was isolated from sugarcane, which contained a 3 183 bp open reading frame (ORF), encoding 1 060 amino acids. Its Genbank accession no. EU 269038. Relative expression of SPSⅡ gene was determined in the different tissues of sugarcane at different time of sugar accumulation by real-time PCR. The results showed highest relative expression of SPSⅡ gene in the canes at early stage during accumulation process of sucrose, but highest in the leaves at middle stage. In the same photosynthetic tissue, relative expression of SPSⅡ was higher at early stage during accumulation process of sucrose than those at middle stage and later stage. Fig 6, Ref 11

References

1 Zhang MF (张明方), Li ZL (李志凌). Sucrose-metabolizing enzymes in higher plants. Plant Physiol Commun, 2002, 38 (3): 289~295
2 Lei MH (雷美华), Ye BY (叶冰莹), Wang BM (王冰梅), Huang ZX (黄祖新), Zhuang H (张华), Xu LP (许莉萍), Chen YQ (陈由强), Chen RK (陈如凯). Cloning of sucrose synthase gene from sugarcane (Saccharum officenarum L.). Chin J Appl Environ Biol (应用与环境生物学报), 2008, 14 (2): 177~179
3 Zhou P (周平), Ye BY (叶冰莹), Chen YQ (陈由强), Chen RK (陈如凯). The recent advances on sucrose phosphate synthase. Lett Biotechnol (生物技术通讯), 2006, 17 (6): 1001~1003
4 Liu NX (刘凌霄), Shen FF (沈法富), Lu HQ (卢合全), Han QD (韩庆点), Liu YG (刘云国). Research advance on sucrose phosphate synthase in sucrose metabolism . Mol Plant Breeding (分子植物育种), 2005, 3 (2): 275~281
5 Li W (李雯), Shao YZ (邵远志), Chen WX (陈维信). Research advances in the relationships of sucrose phosphate synthase (SPS) with fruit quality and maturity and senescence. Acta Bot Bor-occid Sin (西北植物学报), 2005, 11: 2340~2344
6 Yang GZ (杨国志), Zhang MF (张明方). 植物蔗糖代谢参与酶的表达及调控. 北方园艺, 2006, 5: 45~47
7 Castleden K, Naohiro A , Gillespie VJ, MacRae EA, Quick WP, Buchner P, Foyer CH, Furbank RT, Lunn JE. Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses. Plant Physiol, 2004, 135 (3): 1753~1764
8 He WJ (何文锦), Huang JC (黄金存), Zhou P (周平), Ye BY (叶冰莹), Chen YQ (陈由强), Chen RK (陈如凯). Cloning and sequence analysis of sucrose phosphate synthase cDNA from sugarcane leaf . Subtrop Agric Res (亚热带农业科学), 2007, 3 (4): 294~296
9 Lutfiyya LL, Xu NF, Ordine RL, Morrell JA, Miller PW, Duff SMG. Phylogenetic and expression analysis of sucrose phosphate synthase isozymes in plants. Plant Physiol, 2007, 164 (7): 923~933
10 Park JY,Canam T, Kang KY, Ellis DD,Mansfield SD. Over-expression of an arabidopsis family A sucrose phosphate synthase (SPS) gene alters plant growth and fibre development. Transgenic Res, 2008, 17 (2): 181~192
11 Grof CPL, So CTE, Perroux JM, Bonnett GD, Forrester RI. The five families of sucrose-phosphate synthase genes in Saccharum spp. are differentially expressed in leaves and stem. Funct Plant Biol, 2006, 33: 605~610

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Last Update: 2011-10-25