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Regeneration of Plantlets from Mature Endosperm of Jatropha curcas L. and Analysis of Their Stomata(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2011 03
Page:
353-358
Research Field:
Articles
Publishing date:

Info

Title:
Regeneration of Plantlets from Mature Endosperm of Jatropha curcas L. and Analysis of Their Stomata
Author(s):
ZHU Xihong;  LIU Jian;  ZHENG Xiaojiang;  XU Ying;  CHEN Fang
(College of Life Sciences, Sichuan University, Chengdu 610064, China)
Keywords:
Jatropha curcas L.;  callus induction;  adventitious bud differentiation;  leaf differentiation;  rooting;  plantlet regeneration;  stomatal analysis
CLC:
Q943.1 : Q949.753.505
PACS:
DOI:
10.3724/SP.J.1145.2011.00353
DocumentCode:

Abstract:
An effective method for endosperm culture of Jatropha curcas L. was established to develop endosperm plantlets and their stomata were analyzed. Vigorously growing calli were obtained from mature endosperm of J. curcas cultured on Murashige and Skoog (MS) medium containing 2.0 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) at the 7th day under 25 ℃, 12 h light/dark conditions, and a maximum of 89.29% of callus induction frequency was attained. The calli became kelly and had a compact shape when cultured on modified MS medium supplemented with 6-benzyladenine (BAP). Shoot buds were produced when the calli were subcultured on a woody plant medium (WPM) with 1.5 mg L-1 zeatin (ZT) and 0.25 mg L-1 indole-3-acetic acid (IAA), and the rate of shoot differentiation was 32.50%. The regenerated shoots were detached from the calli and transferred to the elongation medium supplemented with indole-3-butyric acid (IBA), BAP and gibberellic acid (GA3). The inoculated leaves from shoot buds regenerated on MS medium supplemented with 0.1 mg L-1 IBA, 0.5 mg L-1 BAP and 0.5 mg L-1 thidiazuron (TDZ). The highest shoot forming rate (85.2%) was obtained on the medium supplemented with 0.25 mg L-1 IAA, 0.5 mg L-1 kinetin (KT), 1.0 mg L-1 BAP and 0.25 mg L-1 GA3. The addition of 0.1 mg L-1 IBA to the 1/2MS medium helped form roots, and approximately 37.5% of the shoots produced five roots. Stomatal analysis showed that the leaves from endosperm-derived plantlets had larger guard cells and lower density of stomata compared with their parent plantlets. Fig 2, Tab 6, Ref 24

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