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Isolation and Identifaction of Acidithiobacillus thiooxidans with Strong Phosphorous Ore Bioleaching Ability(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2011 03
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Isolation and Identifaction of Acidithiobacillus thiooxidans with Strong Phosphorous Ore Bioleaching Ability
LÜ Zaosheng GUAN Haiyan LI Lingling  JIA Weiwei
(College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, China)
Acidithiobacillus thiooxidans strain screening 16S rDNA bioleaching pyrite

Acidophilic sulfur-oxidizing bacterium HY-01 was isolated from mine soil. Strain HY-01 was motile, gram-negative, rod-shaped and 0.5 to 0.7 μm × 1 to 2 μm in size. The strain grew optimally with initial pH 2.0~2.5 and temperature 37 ℃. It could grow with potassium nitrate, ammonium nitrate, ammonirm carbonate, ammonium dihydrogen phosphate, ammonium sulfate and tryptone as nitrogen sources. It could use elemental sulfur and pyrite as energy sources, but not organic minerals and inorganic minerals, including sodium thiosulfate and ferrous sulfate. The morphological, biochemical and physiological characteristics of the strain and the analysis based on 16S rDNA gene sequence indicated that strain HY-01 was most closely related to Acidithiobacillus thiooxidans. The sulfur-oxidizing bacteria could positively increase the dissolution rate and percentage of phosphorus recovery from phosphate ore. Fig 6, Tab 1, Ref 18


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Last Update: 2011-06-23