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Changes in Correlated Cytokines and IgG of Blood Plasma of Piglets under Cold Stress(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2010 05
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Changes in Correlated Cytokines and IgG of Blood Plasma of Piglets under Cold Stress
JI HongYANG HuanminLI ShizeCHEN Ping
(Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China)
cold stress piglet cytokine IgG ELISA glucocorticoid

The changes in correlated cytokines and IgG of blood plasma of piglets under cold stress and the correlation among them were studied, and eighteen Yorkshire piglets aged 45 d were randomly divided into 3 groups. Group 1 (Control, C) was kept at 18~20 ℃, group 2 (Cold exposure, CS) kept at 4~8 ℃ for 12 hours, and group 3 (Cold exposure + RU486, CSRU) was disposed by hypodermic injection (20 mg kg-1 weight) and kept at 4~8 ℃ for 12 hours. Blood samples were collected at 0 h, 1 h, 3 h, 6 h, 9 h, 12 h from superior vena cava of the piglets. The levels of IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ and IgG were measured by method of double antibody sandwich ELISA. The results showed that the effects of cold stress on IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ and IgG obviously differed between group CS and group CSRU. The levels of IL-2, IL-4, IL-6, IL-10 and IFN-γ of blood plasma in group C and group CSRU had a trend of firstly upgrading and then fluctuantly descending, but the elevated speed of group CSRU was faster than that of group CS. The levels of IgG and TNF-α of blood plasma in group CS and group CSRU had a trend of firstly descending and then upgrading, but the change of group C appeared obvious and the others had no change. It is concluded that there are interaction and interdependence relationship among those cytokines, which could rapidly lead to immune response, and glucocorticoids in the immune system plays a significant role. Fig 1, Tab 3, Ref 16


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Last Update: 2010-10-25