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Protection of Zebrafish Against Dichlorvos by Rhodobacter sphaeroides(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2010 03
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Protection of Zebrafish Against Dichlorvos by Rhodobacter sphaeroides
HAN Qingli ZHAO Zhirui LI Yuan WANG Qian WANG Dong QI Hongyan BAI Zhihui
(1College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China)
(2Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China)
(3Fundamental Department, Southwest Forestry College, Kunming 650224, China)
(4Zhongke-Huoli (Beijing) Biotechnology Corporation, Beijing 100012, China)
dichlorvos DDVP zebrafish acute toxicity Rhodobacter sphaeroides biodegradation
X172 : X174

Dichlorvos (DDVP) is one of widely used organophosphate insecticides, with high water solubility and high risk to aquatic animals. In order to explore the detoxification of poisoned fish by DDVP using degrading strains, the acute toxicity of DDVP to zebrafish (Brachydanio rerio) was determined, and the detoxification by using a strain of Rhodobacter sphaeroides with high degradation capability to DDVP was investigated. The result showed that the half lethal concentration (LC50) of DDVP to zebrafish within 24, 48, 72 and 96 h were 42.9, 37.5, 30.7 and 2.6 mg L-1, respectively. The safe concentration was 2.56 mg L-1 which showed that DDVP belonged to low poisonous pesticide to zebrafish. Adding R. sphaeroides (5×107 CFU mL-1) to 28.3 mg L-1 and 33.6 mg L-1 of DDVP-exposed fish water reduced zebrafish mortality significantly from 63.3% and 96.7% both to 0 within 96 h. The degradation of DDVP was detected by HPLC in fish water with and without R. sphaeroides respectively. The result showed that the detoxification by R. sphaeroides was mainly due to DDVP degradation without poisonous degradation products to fish. Fig 3, Tab 1, Ref 19


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Last Update: 2010-06-23