|Table of Contents|

Protection of Zebrafish Against Dichlorvos by Rhodobacter sphaeroides(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2010 03
Page:
358-362
Research Field:
Articles
Publishing date:

Info

Title:
Protection of Zebrafish Against Dichlorvos by Rhodobacter sphaeroides
Author(s):
HAN Qingli ZHAO Zhirui LI Yuan WANG Qian WANG Dong QI Hongyan BAI Zhihui
(1College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China)
(2Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China)
(3Fundamental Department, Southwest Forestry College, Kunming 650224, China)
(4Zhongke-Huoli (Beijing) Biotechnology Corporation, Beijing 100012, China)
Keywords:
dichlorvos DDVP zebrafish acute toxicity Rhodobacter sphaeroides biodegradation
CLC:
X172 : X174
PACS:
DOI:
10.3724/SP.J.1145.2010.00358
DocumentCode:

Abstract:
Dichlorvos (DDVP) is one of widely used organophosphate insecticides, with high water solubility and high risk to aquatic animals. In order to explore the detoxification of poisoned fish by DDVP using degrading strains, the acute toxicity of DDVP to zebrafish (Brachydanio rerio) was determined, and the detoxification by using a strain of Rhodobacter sphaeroides with high degradation capability to DDVP was investigated. The result showed that the half lethal concentration (LC50) of DDVP to zebrafish within 24, 48, 72 and 96 h were 42.9, 37.5, 30.7 and 2.6 mg L-1, respectively. The safe concentration was 2.56 mg L-1 which showed that DDVP belonged to low poisonous pesticide to zebrafish. Adding R. sphaeroides (5×107 CFU mL-1) to 28.3 mg L-1 and 33.6 mg L-1 of DDVP-exposed fish water reduced zebrafish mortality significantly from 63.3% and 96.7% both to 0 within 96 h. The degradation of DDVP was detected by HPLC in fish water with and without R. sphaeroides respectively. The result showed that the detoxification by R. sphaeroides was mainly due to DDVP degradation without poisonous degradation products to fish. Fig 3, Tab 1, Ref 19

References

1 Yang XL (杨先乐), Zhan J (湛嘉), Huang YP (黄艳平). Progress on research of toxic effect of organophosphorous pesticides on aquatic organism. J Shanghai Fish Univ (上海水产大学学报), 2002 (4): 378~382
2 Gan Q, Singh RM, Wu T, Jans U. Kinetics and mechanism of degradation of dichlorvos in aqueous solutions containing reduced sulfur species. Environ Sci & Technol, 2006, 40 (19): 5717~5723
3 Zhang Q, Qu X, Wang W. Mechanism of OH-initiated atmospheric photooxidation of dichlorvos: a quantum mechanical study. Environ Sci & Technol, 2007, 41: 6109~6116
4 Liu C, Qiang ZM, Adams C, Tian F, Zhang T. Kinetics and mechanism for degradation of dichlorvos by permanganate in drinking water treatment. Water Res, 2009, 43: 3435~3442
5 U.S. Environmental Protection Agency. Interim Reregistration Eligibility Decision for Dichlorvos (DDVP). Washington D.C., USA, 2006. 136~137
6 Gao JJ, Liu LH, Liu XR, Zhou HD, Lu J, Huang SB, Wang ZJ. The occurrence and spatial distribution of organophosphorous pesticides in Chinese surface water. Bull Environ Contam & Toxicol, 2009, 82: 223~229
7 Deng MJ (邓敏捷), Wu NF (伍宁丰), Liang GY (梁果义), Chu XY (初晓宇), Yao B (姚斌), Fan YL (范云六). Cloning and expression of ophc2, a new kind of organophosphorus degradation enzyme gen. Chin Sci Bull (科学通报), 2004, 49 (11): 1068~1072
8 Zhao K (赵凯), Yu Y (于影), Jiang D (姜丹), Wang D (王栋), Li ZM (李祖明), Huang GZ (黄国忠), Bai ZH (白志辉). Degradation of dichlorvos by Rhodobacter sphaeroides. Environ Sci (环境科学), 2009, 30 (4): 1199~1204
9 Cui KQ (崔克群). Biostatistics. 2nd ed. Beijing, China: China’s Science and Technology Publishing House (北京: 中国科学技术出版社), 1994
10 Zhu PL (朱蓓蕾). Environmental Toxicology. Shanghai, China: Shanghai Science and Technology Publishing House (上海: 上海科学技术出版社), 1989
11 Li R (李蓉), Zhou GM (周光明), Wang SY (王绍云), Wang L (王丽). Determination of DDVP residues in vegetables with RP-HPLC. J Guiyang Med Coll (贵阳医学院学报), 2006, 31 (1): 58~60.
12 Ren ZM, Zha JM, Ma M, Wang ZJ, Gerhardt A. The early warning of aquatic organophosphorus pesticide contamination by on-line monitoring behavioral changes of Daphnia magna. Environ Monit & Assess, 2007, 134: 373~383
13 Varó I, Navarro J C, Nunes B, Gulihermino L. Effects of dichlorvos aquaculture treatments on selected biomarkers of gilthead sea bream (Sparus aurata L.) fingerlings. Aquaculture, 2007, 266: 87~96
14 Huang ZY (黄周英), Xie JJ (谢进金), Chen LQ (陈琳钦). The acute toxicity of dichlorvos to carassius auratus. J Health Toxicol (毒理学杂志), 2005, 19 (4):.311~312
15 Geng BR (耿宝荣), Yao D (姚丹), Yang YM (杨月梅), Wang YZ (王一铮), Xue QQ (薛清清), LianY (连迎). Toxicity of peticide dichlorvos to Bufo melanostictus tadpoles. Chin J Appl Environ Biol (应用与环境生物学报), 2005, 11 (5): 575~579
16 Cai DJ (蔡道基), Yang PZ (杨佩芝), Cong RZ (龚瑞忠). Experimental Criterion for Environmental Safety Assessment of Chemical Pesticide. Beijing, China: China Environmental Science Press (北京: 中国科学出版社), 1989
17 Khandelwal GD, Wedzicha BL. Reaction of dichlorvos, dichloroacetaldehyde and related compounds with nucleophiles and phenols. Food Chem, 1998, 61 (1~2): 191~200
18 Schramm JD, Hua I. Ultrasonic irradiation of dichlorvose decomposition mechanism. Water Res, 2001, 35 (3): 665~674
19 Shen JY (沈锦玉), Yin WL (尹文林), Liu W (刘问), Shen ZH (沈智华), Qian D (钱冬), Cao Z (曹铮), Wu YL (吴颖蕾). Improvement of fisheries water quality and fish growth of photosynthetic bacteria HZPSB. Bull Sci & Technol (科技通报), 2004 (6): 481~484

Memo

Memo:
-
Last Update: 2010-06-23