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An Improved SDS Acid Phenol Method for Total RNA Extraction from Plant Tissues Rich in Lipid(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2009 02
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An Improved SDS Acid Phenol Method for Total RNA Extraction from Plant Tissues Rich in Lipid
LUO Shaoyin GAO Jihai YE Shengliang XU Ying** & CHEN Fang
(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, School of Life Sciences, Sichuan University, Chengdu 610064, China)
total RNA Jatropha curcas oil SDS acid phenol method endosperm

The total RNA extraction from plant tissues richin lipid by conventional methods often cannot meet the requirement of downstream experiments. This problem was overcomed by modifying Lin’s twice-extraction method: Adjusting the concentration of SDS and pH value of phenol to 1% and 4.0 respectively, and removing the solution II treatment. The yield was high and reached 74.7 μg (100 mg)-1 (fresh weight). There was no genomic DNA contamination in the process. The value of A260 nm/A280 nm was 2.026 and that of A260 nm/A230 nm was 2.082, meaning that protein and other contaminations were low. A 927 bp fragment of Jatropha curcas’ curcin gene was successfully amplified from the obtained total RNA. Compared to Lin’s method, this improved SDS acid phenol method is economical, convenient and has a wide applicability. Fig 5, Tab 1, Ref 13


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Last Update: 2009-05-05