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Analysis of Proteins Interacted with OIPK by Yeast Two-hybrid Method(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2010 04
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Analysis of Proteins Interacted with OIPK by Yeast Two-hybrid Method
YANG Jinli ZHAO Xiaoming YIN Heng ZHANG Hongyan DU Yuguang
(1Biotechnology Department, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China)
(2Graduate School of the Chinese Academy of Sciences, Beijing 100049, China)
yeast two-hybrid Ser/Thr protein kinase plant resistance oligochitosan OIPK

In our previous study, an oligochitosan-induced protein kinase (OIPK) was identified to be involved in oligochitosan-induced defense reactions in tobacco. Two alternatively spliced variants of OIPK gene, referred to as OIPKL and OIPKS, were found in tobacco and OIPKL was the longest one. To further investigate the function of OIPK in the signal transduction pathway of oligochitosan induced plant resistance, yeast two-hybrid screening was performed to find out its interaction partners. OIPKL cDNA was inserted downstream of the DNA-binding domain of the bait vector pGBKT7 and the recombinant pGBKT7-OIPKL plasmid was used as a bait plasmid to screen a tobacco By-2 cell suspension library constructed in pACT2 vector. Four proteins were identified to be interacted with OIPKL protein in the yeast two-hybrid system, including S-adenosyl-L-methionine synthetase, ubiquitin-activating enzyme E1, pyruvate kinase and peroxidase. The results provide evidence for the mechanism of oligochitosan induced plant resistance. Fig 3, Ref 26


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Last Update: 2010-08-25