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Application of molecular biological techniques for analysis of nitrifying communities in wastewater treatment(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2010 01
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Application of molecular biological techniques for analysis of nitrifying communities in wastewater treatment
LI Lei ZENG Wei ZHANG Yue YANG Yingying
(College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China)
nitrifier community molecular biological technique fluorescent in situ hybridization (FISH) polymerase chain reaction (PCR) stable isotope probing (SIP) biological wastewater treatment
X703.1 : X172

Molecular biological techniques have provided a cultivation-independent means for the identification of environmental microorganisms. The molecular biological techniques based on fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) were introduced, including FISH-MAR, FISH-microelectrodes, Clone-FISH, SIP, PCR-DGGE-cloning-sequencing, PCR-T-RFLP, real-time fluorescent quantitative PCR and RT-PCR. The mechanisms and applications of these techniques in the investigation of nitrifier communities in wastewater treatment systems were studied. By using these techniques, the nitrifier communities could be identified in the systems, and the correlation between the dynamic variation in the nitrifier communities and the operating parameters of the systems could be established, and the real state of the system operation could also be analyzed and proved directly and reliably. The results from this study provide theoretical supports for the long-range stable operation of the wastewater treatment systems, and this paper puts forward some suggestions for the future application of those techniques in this field. Fig 5, Tab 1, Ref 53


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Last Update: 2010-02-09