|Table of Contents|

Study on Mutation Type of HMW-GS Component in transgenic wheat progenies(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2010 01
Page:
54-57
Research Field:
Articles
Publishing date:

Info

Title:
Study on Mutation Type of HMW-GS Component in transgenic wheat progenies
Author(s):
REN Yanping ZHU Changqing QIN Jianbing
(Research Division of Molecular Biology and Informatics, Xinjiang Normal University, Urumqi 830053, China)
Keywords:
mutation type SDS-PAGE transgenic wheat glutenin allelic variation
CLC:
Q78 : S512.103.3
PACS:
DOI:
10.3724/SP.J.1145.2010.00054
DocumentCode:

Abstract:
Mutation types of HMW-GS component in independent transgenic wheat lines were investigated in this paper. The results showed that there were 21 categories in the 1 000 selected randomly of B72-8-11b, the expression of 1Dx5 gene accounted for 49.4%, three types were detected on Glu A1, and N subunit was the major types with frequency of 94.7%, thirteen types were detected on Glu B1, and 17+18 subunit was the major types with frequency of 61.5%, nine types were detected on Glu D1, and N subunit was the major types with frequency of 49.7%; There were 11 categories in the B102-1-2, the expression of 1Ax1 gene accounted for 95%, two types were detected on Glu A1, and N subunit was the major types with frequency of 95%, seven types were detected on Glu B1, and 17+18 subunit was the major types with frequency of 98.7%, three types were detected on Glu D1, and N subunit was the major types with frequency of 98.3%. The results also indicated that the transmission of the transgene and inheritance of its expression followed Mendelian ratios with the method of quantitative genetics, and T7 generation lines almost homozygous. It would be beneficial for deciding the selection strategy in breeding program. Fig 2, Tab 4, Ref 9

References

1 Qin JB (覃建兵), Zhu CQ (祝长青), Zhao HX (赵惠新). Measures of interesting gene 1Dx5 expression in transgenic wheat progenies. J Huazhong Norm Univ (华中师范大学学报), 2008, 42 (2): 267~271
2 Li SH (李三和), Li J (李举), Wang NL (王娜丽), He GY (何光源). Variation of HMW-GS expresssion resulted by the foreign 1Dx5 gene. Lett Biotechnol (生物技术通讯), 2007, 18 (2): 217~219
3 Wang NL (王娜丽), Wang YS (汪越胜), Li J (李举), Li SH (李三和), He GY (何光源). Studies on transgenes 1Dx5 and 1Ax1 transferred into elite wheat cultivars by cross breeding. J Wuhan Bot Res (武汉植物学研究), 2007, 25 (4): 331~334
4 Huang DY, Khan K. Quantitative determination of high molecular weight glutenin subunit of hard red spring wheat by SDS-PAGE: Quantitative effects of total amounts on bread making quality characteristics. Cereal Chem, 1997, 76: 781~788.
5 Zhang XM, Xu HJ, Du LP, Ye XG, Xin ZY, Guo AG, Xue S, Ma YZ. Excision of bar gene from transgenic wheat obtained by biolistic co-transformation. Acta Agron Sin, 2004 , 30 (1): 26~30
6 He G Y, Rooks L, Steele H S, Bekes F, Gras P, Tatham A S, Fido R, Barcelo P, Shewry P R, Lazzeri P A. Transformation of pasta wheat (Triticum turgidum L. var. durum) with high-molecular-weight glutenin subunit genes and modification of dough functionality. Mol Breeding, 1999, 5: 377~386
7 Wang YS (汪越胜), Qin JB (覃建兵), Chang JL (常俊丽), Fang JY (房敬业), He GY (何光源), Peter Shewry. Study on heritance of foreign gene 1Dx5 expressed in transgenic wheat. Mol Plant Breeding (分子植物育种), 2005, 3 (4): 451~455
8 Wang YS (汪越胜), He T (柯涛), Li SH (李三和), Qin JB (覃建兵), Liu Y (刘勇), He GY (何光源). Variation of HMW-GS component in the progeny of transgenic wheat (Triticum aestivum L.). Acta Agron Sin (作物学报), 2005, 31 (4): 529~531
9 Pan ZF (潘志芬), Deng GB (邓光兵), Liu Y (刘毅), Wu F (吴芳), Yu MX (余懋群). Composition of high molecular wheat glutenin subunits and effect on wheat baking quality. Chin J Appl Environ Biol (应用与环境生物学报), 2006, 12 (2): 155~159

Memo

Memo:
-
Last Update: 2010-02-09