|Table of Contents|

PREPARATION AND CHARACTERIZATION OF FeMo COFACTORS CONTAINING SINGLE AND DOUBLE Mo FROM NITROGENASE(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1997 03
Page:
258-262
Research Field:
Publishing date:

Info

Title:
PREPARATION AND CHARACTERIZATION OF FeMo COFACTORS CONTAINING SINGLE AND DOUBLE Mo FROM NITROGENASE
Author(s):
Huang Heqing Zhang Fengzhang Qiu Xuehui Li Qingmei
1. Department of Biology, Xiamen University, Xiamen 361005;
2. Research Center of Environment Science, Xiamen University, Xiamen 361005
Keywords:
NitrogenaseFeMo cofactorFe-S clusters containing single and double Mospectral analysis
CLC:
-
PACS:
DOI:
-
DocumentCode:

Abstract:
Preparation of FeMo cofactors containing single and double Mo was related with basicity of N-methylformamide. The mininal elemental composition of FeMo cofactor containing single and double Mo was found to be Mo: 6Fe: 6S by using inductively coupled plasma and proton induced X-ray emission analytical technique. F6Mo cofsctors containing single and double Mo showed featureless absorbence peak in the ultraviolet and visible spectral range and did not contain homocitrate.

References

1. Watt GD. Burns A. Tennent DL. Stoichiometry and spectral properties of the MoFe cofactor and noncofactor redox centers in the MoFe protein of nitrogenase from AwWbacler vmelanGn. Biochemisery. 1981,201:7272~7277
2. Watt GD, Huang HQ, Reddy KRN. Redox properties of the nitrogenase protein. Molebdennm Enzymes cofactor and Model Systems. 1993, 535:243~256
3. Shah VK,Brill WJ.lsolation of iron-molybdenum cofactor from nitrogenase. Proc. Nall Acda Sci USA. 1977, 74: 3249~3253
4. Burgess BK. The iron-molybdenum cofactor of nitrogenase. Chem. Rev. 1990.90:1377~1460
5. Yang SS, Pan WH, Friesen GD. lton-molybdenum cofactor from nitrogenase. J Biol Chem. 1982, 257:8042~8048
6. Huang HQ, Kofford M, Watt GD et al. Purification, composition, charge, and molecular weigth of FeMo cofactor from Azolobacter vinelandii nitrogenase. J Inorgan.Biochem. 1993, 52:59~75
7. 黄河清,Watt GD.固氮酶铁钼辅基理化特性的研究.生物化学杂志.1995. 11(4):465~469
8. Smith BE, Rady RR.Metalloclusters of the nitrogenase. Eur J Biochem. 1985. 205:1~15
9. Huang HQ,Watt RK, Frankel RK et al.Role of phosphate in Fe(11) binding to horse spleen ferritin. Biochemistry, 1993, 32:1681~1687
10. 黄河清,张凤章,许良树等.细菌铁蛋白铁释放动力学研究.生物物理学报.1996.12(1):37~42
11. 张凤章,尹蓓,黄河清等,高柠橡酸盐对固氮酶铁钼辅基重组活性的影响、厦门大学学报(自然科学版).1996,35(1):100~106
12. 刘国诠等主编·生物工程下游技术·北京:化学工业出版社,1993
13. Kim JS、Rees DC. Structure models for the metal centers in the nitrogenase molybdeum-iron protein. Science, 1992. 257: 1677~1682

Memo

Memo:
-
Last Update: 1900-01-01