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EFFECT OF PROTEINASE INHIBITORS ON THE MIDGUT PROTEINASE OF CLOSTERA ANACHORETA LARVAE(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1997 03
Page:
240-245
Research Field:
Publishing date:

Info

Title:
EFFECT OF PROTEINASE INHIBITORS ON THE MIDGUT PROTEINASE OF CLOSTERA ANACHORETA LARVAE
Author(s):
Bian Xueyu J. T. Christeller B. D. Shaw Han Yifan
1. Research Institute of Forestry, CAF. Beijing 100091, P. R. China;
2. Horticulture and Food Research Institute of New Zealand, Private Bag 11030, Palmerston North, New Zealand
Keywords:
Clostera anachoretaNotodontidaeLepidopteranmidgutproteinase inhibitorserine proteinase
CLC:
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PACS:
DOI:
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DocumentCode:

Abstract:
The major proteinase activities in the larval midgut of Clostera anachoreta were characterized. Overall digestive capacity which was measured by casein hydrolysis showed a alkaline pH optimum and serine proteinase activity. The abences of cysteine, aspartic acid and metallo-proteinases were confirmed by specific proteinase inhibitors. Trypsin,chymotrypsin, leucine aminopeptidase and elastase-like activities were detected using specific p-nitroanilide synthetic substrates.These enzyme activities also showed pH optima in the alkaline range. The dominant proteinase in the midgut was elastase. 23 serine proteinase inhibitors were screened and varied widely in their ability to inhibit casein hydrolysis. potato proteinase inhibitor 1 and pumpkin fruittrypsin inhibitor were the most effective,and represent the possible candidates for gene transformation to produce plants tolerant to this pest.

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