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DIVERSITY OF SYMBIOTIC GENE LOCATION AND SYMBIOTIC EFFECTIVENESS OF RHIZOBIUM HUAMIII STRAINS(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1997 01
Page:
49-54
Research Field:
Publishing date:

Info

Title:
DIVERSITY OF SYMBIOTIC GENE LOCATION AND SYMBIOTIC EFFECTIVENESS OF RHIZOBIUM HUAMIII STRAINS
Author(s):
Zou Xianghong Li Fudi Cao Yanzhen Chen Wenxin Chen Huakui
1. College of Biological Science, Chinese Agricultural University, Beijing 100094;
2. Department of Microbiology, Huazhong Agricultural University, Wuhan 430070
Keywords:
Rhizobium huakuiisymbiotic genesymbiotic effectiveness
CLC:
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DOI:
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Abstract:
Forty strains of Rhizobium huakuii were isolated from different nodules on the same heat plant (1 isolate from 1 nodule). Intrinsic antibiotic resistance (IAR) of all strains was tested with 10 antibiotics and they could be classified into 22 IAR clusters. The plasmid profiles of all strains were examined by using a mobified Eckhard procedure of plasmid visualization. The number of the strains varied from 1 to 4, and their M, were estimated at 83 to 226 Mu in comparison with the plasmids of strain USDA205 as reference. The plasmid profiles of all strains could be classified in to 6 different plasmid types by plasmid number and weight. All the plasmids were hybridized with nod ABC and nif HDK and the results showed that the symbiotic genes were located on Chromosome in the strain with one plasmid, and the strains with other plasmid types harbored one sym plasmid besides other plasmid(s). The Mt of the sym plasmids varied from 117 to 226 Mu. Symbiotic effectiveness of typical strains with different plasmid type was determined and the results indicated that their symbiotic effectiveness varied considerably. The symbiotic effectiveness of the strain with one plasmid was the lowest. The symbiotic effectiveness of the strains of the sixth plasmid type was the highest, but the numberof the strains was only a small portion in all the strains tested (7.5%).

References

1 Mozo T,Cabrera E,Ruiz-Agrueso T. Diversity of ptasmfd profiles and conservation of symbiotic nitrogen genes newly isolated Rhizobiwa strains nodulating aulla. Apl Bavivoa Microbiol. 1988,54.1262-1267
2 Laguerre G,Genieux E,Amarger N. Conformity and diversity among field isolates of Rhiaabiam &pamiaoearwn bv.rinm,bv.trifolii and bv.phaeeoli revealed by DNA hybridization using chromosome and piasmid probes. Can J Microbiol. 1993,39,412-419
3 Chen W X,U G S,Li Y et al. Rhizobium huakuii sp. nov. Isolated from the root nodules of Aeropdaa eiaieas. fat J Syst Boeterid.1991,41,275-280
4 邹向宏,曹燕珍,李阜棣.萦云英根启,天然坑药性和质位研究.华中农业大学学报.1994,13,325-331
5 Echhardt T. A rapid method for the Identification of plasmid deoxyribonuleic acid in bacteria. Plaamid. 1978,1.584-588
6 Masterdt RV,Prakash PK,Atherly AG. Conversation of symbiotic nitrogen fixation gene sequences in R.tosium and Bradf-Rhizo-bism japosieam. J Bwleriol. 1985,163, 21-26
7 金润之.萦云英根启菌Ra159的巨大质控上存在有nod和nif基因的证明.微生物学报.1993,33,170-176
8 魏辉,李草棣.萦云英根瘤菌携带共生基因的物理证据.华中农业大学学报.1989,8,12-14
9 Sober-Chavez G,Na jars R,Calve E et al. Partral dektbn of the Rhiwbium Leguminosarum CFN23 symbiotic plasmid implied a ampLication of plasmid DNA sequence. Mat Mierobiol. 1991,5.89-95
10 Galas DJ, Chandler M. Bacterial Insertion sequences. In: Berg DE, Houe M ed.Mobile DNA,109-162. Washington DC, America Society for Microbiology
11 Harrison SP,Jona DG,Schunmann PHD et al.Variation in Rhimbiam kpamuoaarum bv. trifolii sym plasmids and the association with efectivensess of nitrogen fixation. J Oes AficroUol. 1988,13482721-2730
12 Hyne MF,McGregor N F. Two plasmids other than the nodulation plasmid are necessary for formation of nitrogen fixing nodules by Rhizobium leguminosarum Mol Micrabiol. 1990,4,407-414
13 Susana Brom,Alejandro G S,Tomasz S d al. Different plasmids of Rkimbium lepumuoasrum bv.phaseoli are required for optimal symbiotic performance. J Back-riot. 1992,174,5183-5189
14 叶政.增强快生型大豆根启菌共生困篮活性.武汉大学学报.1990,生物工程专刊:34-36

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