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GROWTH CHARACTERISTICS AND FATTY ACID ANALYSES OF BRADYRHIZOBIUM STRAINS ISOLATED FROM THE ROOT NODULES OF PEANUT(ARACHIS)(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1997 01
Page:
44-48
Research Field:
Publishing date:

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Title:
GROWTH CHARACTERISTICS AND FATTY ACID ANALYSES OF BRADYRHIZOBIUM STRAINS ISOLATED FROM THE ROOT NODULES OF PEANUT(ARACHIS)
Author(s):
Zhang Xiaoping Li Dengyu Giselle Nick Scott W. Tight Kristina Lindstrom
1. Departsnent of Applied Microbioloigy, Faculty of Agronomy, Sichuan. Agriculture University, Yaan 625000, Sichuan, China;
2. Department of Applied Chemistry and Microbiology Biocentre IA POB27, 00014, Universtiy of Helsinki, Finland;
3. Analytical
Keywords:
Bradyrhizobium sp. (arachis)FAMEphylogeny
CLC:
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PACS:
DOI:
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DocumentCode:

Abstract:
Twenty two rhizobial strains isolated from the root noduled of two Chinese peanut cultivars (Arachis hypogaesa L. Tianfu no. 3 and a local cultivar) growing in four different areas in Sichuan were analyzed and compared with other bradyrhizobial strains form strains from Bradyrhizobium japonicum, B. alkanii and B. intermedium.The results showed that all the 22 rhizobial isolates from the peanut in Sichuan were bradyrhizobia. FAME and two-dimensional analyses on the principal component indicated component indictted that Bradyrhizobium sp. (arachis) was dif ferent from other bradyrhizobia because of the presence of fatty acid 16:1 waid with significantly higher perecntage, but closely related to B. japonicum. The results suggest that resluts suggest that FAME analysis that FAME analysis provides a relatively simple and reliable procedure for the inital characterization of Bradyrhizobium isolates.

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