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STUDIES ON THE DIFFERENTIATIOX AND INDUCTION OF CALLI FROM IMMATURE ENDOSPERMS(HAPLOID) OF SOME GYMNOSVERMS(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1997 01
Page:
19-22
Research Field:
Publishing date:

Info

Title:
STUDIES ON THE DIFFERENTIATIOX AND INDUCTION OF CALLI FROM IMMATURE ENDOSPERMS(HAPLOID) OF SOME GYMNOSVERMS
Author(s):
Qi Liwang Han Suying Yang Yunlong Li Huaizhong Wu Xiaoling
Department of Forestry, Shanxi Agricultural University, Taigu 030801
Keywords:
gymnospermconeendospermheat shockdark culture
CLC:
-
PACS:
DOI:
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DocumentCode:

Abstract:
The immature endosperms of some gymnosperms were cultured as explants on a modified MS medium containing various concentrations and conbinations of hormones, and treated with heat shock, light culture,dark culture and endosperm bruis etc. respectively. The results showed that the bruised endosperms and dark culture were good for inducting calli and after the dark culture for 25~30 d with heat shock, the highestinduction rate of calli from all the tested gymnosperms was 86.4% in a medium supplemented with ρ(NAA)=0.5~0.75 mg/L,ρ(2,4-D) =0.2~0.5 mg/L, and 5% of sucrose. As for Pinus tabulaeforms, P.bungeana, Picea wilsonii and Larix principis rupprechtii, the medium should be supplemented with ρ(KT)=1.0 ~2.0 mg/L, and Picea meyeri Rehd et Wils and Platycladus orientalis, the medium with BA and KT both.

References

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Last Update: 1900-01-01