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A GC-MS METHOD FOR QUANTITATING S-(N-METHYLCARBAMYOL)GLUTATHIONE,A METABOLITE OF N,N-DIMETHYLFORMAMIDE IN BLOOD(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1996 04
Page:
376-381
Research Field:
Publishing date:

Info

Title:
A GC-MS METHOD FOR QUANTITATING S-(N-METHYLCARBAMYOL)GLUTATHIONE,A METABOLITE OF N,N-DIMETHYLFORMAMIDE IN BLOOD
Author(s):
Shen Jianhua W. Kesler
1. Department of Biochemistry and Toxicology, Shanghai Institute of Entomology Academia Sinien Shanghai 200025;
2. National Research Center for Environment and Healthe, Institute of Toxicology, Munich, F.R.G.
Keywords:
NN-dimethylformamidebloodthioestersGC-MS
CLC:
-
PACS:
DOI:
-
DocumentCode:

Abstract:
N,N-dimethylformamide (DMF)is widely used as an orsanic solvent in different chemical processes.The toxicology of DMF is related to its metabolites.The results published in recent years suggest the possible existence of S-(N-methylcarbemoyl) glutathione (SAG) and S-(N-methylcarbamoyl) cystein (SMC) as inter mediate metabolites in mammalians.On the basis of conversion of SMG into ethyl N-methylcarbamate(EMC) that was measured on the OC-MS, a method for quantitatins SAG and other N-methylcarbamoyl thioesters in rat blood with the detection limits of 10 μmole SMG in 1 liter biood plasma was developed.The method was valianted with synthetic SAG and EMC.It took 0.5 mL of bloob plasma for each assay.

References

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Last Update: 1900-01-01