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CLONING AND EXPRESSION OF THE P1 GENE OF TURNIP MOSAIC VIRUS HANGZHOU ISOLATE I(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1996 02
Page:
141-146
Research Field:
Publishing date:

Info

Title:
CLONING AND EXPRESSION OF THE P1 GENE OF TURNIP MOSAIC VIRUS HANGZHOU ISOLATE I
Author(s):
Zhang Yaozhou Gong Bowei
1. Institlte of Biochemistry, Zhejiang Agricultural University, Hangzhou 310029;
2. Shanghai Institute of Cell Biology, Academia Sinica, Shanghai 200031
Keywords:
Turnip mosaic virusP1 geneE. coli expression
CLC:
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PACS:
DOI:
-
DocumentCode:

Abstract:
The turnip mosaic virus Hangzhou isolate I(TuMV H1),wnich infected Brassica naprs more seriously than other TuMV isolates,was isolated in Hangzhou.The P1 gene of TuMV H1 was cloned by reverse transcription PCR and was inserted into pGEMEX-1 fusion expression vector.A fusion protein containing the P1 protein that is Mr≈6×104 was expressed in E. coli.

References

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