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THE SURFACES AND ADHESION OF THE PLANT CELLS CULTURED IN VITRO(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1996 02
Page:
109-114
Research Field:
Publishing date:

Info

Title:
THE SURFACES AND ADHESION OF THE PLANT CELLS CULTURED IN VITRO
Author(s):
Guo Xiaocai Wu Boji Jiang Hui Zheng Guochang
1. Chengdu Institute of Biology, Academia Sinica, Chengdu 610041;
2. Laboratory of Cytobiology, Lanzhou University, Lanzhou 730000
Keywords:
surface of Plant callus cellprotuberance or wartfibrildeposition of wall componentscell adhesioncell dispersivitymorphogenesis
CLC:
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PACS:
DOI:
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Abstract:
The cell surfaces of carrot(Daucus carote)and tobacco(Nicotiana tobacum)cultured in vitro were studied using scanning electron microscopy.Protuberances or warts,in various shapes and sizes and mainly containing pectin,and fibrils occurred on the flee surfaces(or outer surfaces of cell walls)of the plant callus cells.There were groups of mini-warts in the pits on the free surfaces of some cells.The warts could be fused and became big ones or combined and became bridge form,and were often associated with librils.The fibril interwoven,fibril-wart associstion,and wart-interfusion fored the Polymeric networks on the outer surface of the cell wall.It was thought that the deposition and assembly of the primary wall components into a growing wall were carried out mainly by intercalating onto the outer surface of the existing wall.Individual cells,being homogeneous or heterogeneous,could adhere yi3 the interwoven polymeric networks of fibrils and warts.The occurrence of warts on the cell surface,being different from cell to cell,might affect the dispersivity of the cells.Compared with friable white tobacco callus cells from subcultures,there were more warts remarkably on the free surfaces of compact tobaccoo callus cells from white compact calli cocultured with green carrot calli or from pale green compact calli cultured on the regeneration medium. During plant regeneration,the white friable tobacco calli first became compact and pole green,then adventitious buds appeared and plantlets or shoots would resenerate.Thus,it was concluded that the cell adhesion based on the assembly of wall components,charactcrized by the occurrence and interwoven of warts and fibrils on the outer surface of the cell wall,was essential for the cell differentiation and morphogenesis.The significance of the cell adhesion was discussed.

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