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CULTURE OF PROTOPLASTS FROM HYPOCOTYLS AND COTYLEDONS OF ORYCHOPHEAGMUS VIOLACEUS(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1996 01
Page:
8-14
Research Field:
Publishing date:

Info

Title:
CULTURE OF PROTOPLASTS FROM HYPOCOTYLS AND COTYLEDONS OF ORYCHOPHEAGMUS VIOLACEUS
Author(s):
Zhou Jiming Wei Zhiming Liu Shigui Luo Peng
1. National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032;
2. Dept of Biotechnology;
3. Dept of Biology, Sichuan University, Chengdu 610064
Keywords:
Orychopheagmus violaceushypocotylcotyledonprotoplast cultureplant regeneration
CLC:
-
PACS:
DOI:
-
DocumentCode:

Abstract:
Hypocotyl and cotylodon protoplasts were isolated with enzyme solution and their yields were 5×108g-1 and 1×107g-1.After purification with CPW-9M and CPW-21S, they were cultured in MS liquid medium with different hormones at a density of 5×104/mL. The first division occurred on the second day and the division frequency was about 50% and 40% respectively. After two weeks,a great number of cell colonies and calli were produced,and then they were transferred into the solid proliferation medium. About two weeks later, the calli grew into the size of 2mm,then they were moved into the differentistion medium and shoot formation was initiated soon. The shoots were excised and roots were induced in a rooting medium.The frequenCy of plant regeneration was 100%.

References

1. 罗鹏,兰泽遂,黄建等.珍贵油料植物诸葛菜的研究.自然资源学报.1991,6(3):206~210
2. 周冀明,卫志明,刘世贵等. GUS荃因在诸葛菜子叶原生质体中的瞬间表达.应用与环境生物学报.1995,1(1):7~11
3. 许智宏,Davey M R,Cocking E C.高等植物根原生质体的分离和培养.中国科学(B辑).1984,11:1012~1017
4. Cocking E C. A method for the isolation of plant protoplasts and vacuoles. Nature. 1960,187:927~929
5. 程振东,卫志明,许智宏.甘蓝型油菜下胚轴原生质体培养研究.生物工程学报.1994,10(1):30~33
6. Lu DY. Plant regeneration from seedling cotyledon protoplasts. Z. Pflarzenphysiol. 1982,107:59~63

Memo

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Last Update: 1900-01-01