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THE EXPRESSION OF T-cyt GENE IN TRANSGENIC TOBACCO AND REGULATION FOR PLANT DEVEOLPMENT(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1996 01
Page:
1-7
Research Field:
Publishing date:

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Title:
THE EXPRESSION OF T-cyt GENE IN TRANSGENIC TOBACCO AND REGULATION FOR PLANT DEVEOLPMENT
Author(s):
Ma Mi Zhou Dafeng Xu Jiaxi Kuang Tingyun Tang Peisong Lin Zhongping
1. Institute of Botany, Academia Sinica, Beijing 100044;
2. Department of Chemistry, Peking University, Beijing 100871
Keywords:
cytokininT-cyt genepromotergene expressiontransgenic tobacco
CLC:
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DOI:
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Abstract:
The T-cyt gene Coding the key enzyme of cytokinin biosynthesis was placed under control of CaMV 35S,rbc S and T-cyt native promoters respectively.The chimeric plasmids were transferred separatedly into Nicotiana tubacum. The transgenic plants were confirmed by PCR DNA amplification,Southern hybridization and NPT Ⅱ detection.The results of Northern analysis showed that T-cyt gene could express in leaves,stems and roots of tobacco with transgene of caMV 35S/T-cyt. The transcript level of rbc S/T-cyt gene activities in leaves was higher than that of stems and little transcript activity was found in roots of those plants. ELISA indicated that the roots of transgenic tobacco contained more isopenteryl transferase. Moreover, a series of morphological and physiological changal, such as promotion of axillary bud growth, inhibition of root srowth, promotion of chlorophyll synthesis and delay of leaf senescence,were observed.

References

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