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PLANT REGENERATION FROM LONG-TERM SUBCULTURED CALLI OF POTATO(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1995 01
Page:
26-33
Research Field:
Articles
Publishing date:

Info

Title:
PLANT REGENERATION FROM LONG-TERM SUBCULTURED CALLI OF POTATO
Author(s):
Ye Yan Miao Shuhua
Chengdu Institute of Biology, Academia Sinica, Chengdu 610041
Keywords:
potatocallussubcultureplant regeneration
CLC:
-
PACS:
DOI:
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DocumentCode:

Abstract:
The factors effecting callus initiation and growth, as well as plant regeneration from long-term cultured calli of potato(Solanum tuberosum L.) were studied. The frequency of callus initiation from stem segments was higher than that from leaf discs. The frequency of callus initiation by Dicamba was about 90% from stem segments and leaf discs, but that by 2, 4-D was lower, and roots were induced on the explants. It was necessary to supply casein hydrolasate at 0.5 g/L in the regeneration medium. The suitable concentration of sucrose was 2% -4%. The regeneration frequency of long-term cultured calli could reach 80% when ZT 2.0 mg/L and GA0.1mg/L were supplied in the regeneration medium. Of the three tested genotypes.only one could reproduce plants. Furtheremore,secondary calli were also induced and could reproduce plants.

References

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