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TRANSIENT EXPRESSION OF GUS GENE IN COTYLEDEN PROTOPLASTS OF ORYCHOPHRAGMUS VIOLACEUS(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
1995 01
Page:
7-11
Research Field:
Articles
Publishing date:

Info

Title:
TRANSIENT EXPRESSION OF GUS GENE IN COTYLEDEN PROTOPLASTS OF ORYCHOPHRAGMUS VIOLACEUS
Author(s):
Wei Zhiming2 Zhou Jiming1 Liu Shigui2 Luo Peng3
1. National Laboratory of Plant Molecular Genetics of Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032;
2. Depentment of Biotechnology of Sichuan University, Chengdu 610064;
3. Depentment of Biology of Sichuan University, Chengdu 610064
Keywords:
Orychophragmus violaceusprotoplastGUS transient expression
CLC:
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PACS:
DOI:
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DocumentCode:

Abstract:
This paper presents the study on the transient expression level of the GUS reporter gene with cotyleden protoplasts of Orychophragmus violaceus. The highest expression level was obtained when a smaller (5.7 kb)plasmid 25-30 μg, 15% and pH 8.0 of PEG were used. The carrier DNA, ctDNA and heat shock treatment seemed no effect on its expression.

References

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