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Effect of Dark Treatment on Fluorescence Properties of Cytoledon Thylakoid Membranes of Brassica napus L.(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2008 No.01
Page:
24-031
Research Field:
Reviews
Publishing date:

Info

Title:
Effect of Dark Treatment on Fluorescence Properties of Cytoledon Thylakoid Membranes of Brassica napus L.
Author(s):
FU Jingfeng et al.
(Key Laboratory of Bioresources and Ecoenvironment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China)
Keywords:
Keywords Brassica napus L. cotyledon thylakoid membrane dark treatment spectra property
CLC:
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DOI:
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Abstract:
Abstract Shuza10 rapeseed (Brassica napus L.) was grown under natural and dark conditions 7 days after sowing. The cotyledons of untreated rapeseed were harvested on day 5, 7, 14, 17, 21, 24 and 27, and those of the dark treated on day 8, 10, 12, and 14. Thylakoid membranes were prepared from the rapeseed cotyledons of the two treatments, respectively. A comparative study on their pigment composition, absorption spectra at room temperature, chlorophyll emission and excitation fluorescence spectra and protein flourescence spectra was conducted. Compared with the untreated rapeseed, the cotyledon thylakoid membranes treated in dark had much less Chl (Chl a and Chl b), and a lower Chl a/b ratio and chlorophyll/carbtenoid (Chl/Car) ratio. Furthermore, the chlorophyll fluorescence spectra indicated that its capacities of harvesting light and being excited were lower after dark treatment. In addition, the protein composition of cotyledon thylakoid membranes also changed in the dark treatment, suggested by significant difference in protein influorescence. The data indicated that dark treatment induced senescence of rapeseed cotyledon. Fig 10, Ref 23

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Last Update: 2008-03-05