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Comparison of RNA Silencing Inducing Efficiency of Three PVX CP Gene Constructs via Transient Expression(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2007 Vol.13 No.1
Page:
1-4
Research Field:
Reviews
Publishing date:

Info

Title:
Comparison of RNA Silencing Inducing Efficiency of Three PVX CP Gene Constructs via Transient Expression
Author(s):
ZHU Xiaopinget al.
(Laboratory of Plant Virology, College of Plant Protection, Shandong Agricultural University, Tai′ an 271018, Shandong, China)
Keywords:
Keywords RNA silencing potato virus X (PVX) coat protein (CP) gene agroinfiltration virus resistance
CLC:
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DOI:
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DocumentCode:

Abstract:
Abstract In plant, RNA silencing is an adaptive defense mechanism against virus infection, and pathogenderived resistanceis attribute to this mechanism. The size, arrangement and location of transgenes in transgenic plant genome affect the efficiency of RNA silencing inducing. Plant expression vectors with untranslatable transgene arranged as sense, antisense orientation and inverted repeat were constructed with the fulllength potato virus X (PVX) coat protein (CP) gene and their efficiency of RNA silencing inducing was analyzed after transient expression via agroinfiltration. The results showed that the inverted repeat was the most efficient inducer. By agroinfiltration transient expression system, the expression and function of the transgene could be quickly tested, so as to save time and resources. Fig 3, Tab 2, Ref 21

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Last Update: 2007-03-06