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Composition of High Molecular Weight Glutenin Subunits and Effect on Wheat Baking Quality(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2006 Vol.12 No.2
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Composition of High Molecular Weight Glutenin Subunits and Effect on Wheat Baking Quality
PAN Zhifenet al.
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) (2Graduate University of Chinese Academy of Sciences, Beijing 100039, China)
Keywords wheat HMW-GS baking quality SDS sedimentation

Abstract The composition of high molecular weight glutenin subunits (HMW-GSs) of 386 wheat lines from CIMMYT by the SDS-PAGE was identified, the micro SDS sedimentation value was determined, and the effect of the HMW-GS on wheat baking quality was evaluated. The result of the electrophoresis showed that the allelic variations were very rich in these investigated wheat lines and the frequencies of the allelic subunits at each encoding locus were widely different. Totally, there were 14 different subunit bands and 25 types of subunit combinations. At GluA1, subunits Null, Ax1 and Ax2* were observed with the frequencies of 71.50%, 16.32% and 4.15%, respectively. Subunits Bx7, Bx7+By9, Bx7+By8, By22, Bx20, Bx14+By15 and Bx6+By8 were found at loci Glu-B1 with frequencies of 42.75%, 21.24%, 17.36%, 13.47%, 3.89%, 1.04% and 0.26%, respectively. At loci Glu-D1, subunit Dx5+Dy10, Dx2+Dy12, Dx5+Dy12 and Dx2+Dy10 were shown with frequencies 76.42%, 22.00%, 1.04% and 0.52%, respectively. The entries containing subunit pairs Dx5+Dy12 and Dx2+Dy10 were found, and they rarely occurred in wild genotypes and were very important for determining the effect of individual HMW subunit at loci Glu-D1 on baking quality of wheat. Also, allelic variation strongly affected the baking quality of wheat. For the HMW subunits encoded at loci Glu-A1, the contribution of subunit Ax1 was much better than that of Ax2* and Null, and the difference between the latter two was not significant. For that of loci Glu-B1, the contributions of Bx7+By8 and By22 were significantly better than those of the others. At loci Glu-D1, the differences between subunits Dx 5+Dy10 and Dx2+Dy12 were consistent with those reported previously, that is, the contribution of Dx5+Dy10 was greatly larger than that of Dx2+Dy12. Fig 1, Tab 8, Ref 10




Last Update: 2006-04-25