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Extraction and analysis of chloroplast DNA in nonheading Chinese cabbage(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2005 Vol.11 No.3
Page:
283-285
Research Field:
Reviews
Publishing date:

Info

Title:
Extraction and analysis of chloroplast DNA in nonheading Chinese cabbage
Author(s):
SHI Gongjunet al.
(1National Key Laboratory of Crop Genetics & Germplasm Enhancement, 2 College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)
Keywords:
Keywords nonheading Chinese cabbage SDSproteinase method cpDNA extraction
CLC:
-
PACS:
DOI:
-
DocumentCode:

Abstract:
Abstract Chloroplast DNA was extracted from young seedlings of nonheading Chinese cabbage by SDS proteinase method. Its quality was evaluated by agrose gel electrophoresis and UV spectrophotometer. Except the main cpDNA band, two minor particle molecules about 1 375 bp and 831 bp appeared in the profile of the original and new CMS line. RAPD analysis was made with the cpDNA as template and clear amplification profile was obtained. This method was proved to be more efficient, more timesaving and less pollution than traditional methods. Fig 2, Tab 1, Ref 14

References

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Last Update: 2005-06-24