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Plant regeneration from embryogenic cell suspensions via somatic embryogenesis of longan (Dimocarpus longan Lour.)(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
Vol.08 No.5
Page:
485-491
Research Field:
Reviews
Publishing date:

Info

Title:
Plant regeneration from embryogenic cell suspensions via somatic embryogenesis of longan (Dimocarpus longan Lour.)
Author(s):
LAI Zhongxionget al.
(Institute of Subtropical Pomology, Fujian Agriculture and Forestry University, Fuzhou350002, China)
Keywords:
Keywords longan embryogenic suspension plant regeneration somatic embryogenesis
CLC:
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PACS:
DOI:
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DocumentCode:

Abstract:
AbstractThe factors affecting establishment of embryogenic suspensions from embryogenic calli, the methods of suspension maintenance and plant regeneration via somatic embryogenesis of longan were studied. The results showed that the key factors for establishing an excellent embryogenic suspension were as follows: initiating from friable embryogenic callus in the liquid medium with the same components as the subculture medium of friable embryogenic callus free of agar; maintaining suspensions via alternative culture of the media amended with 5 mg L-1 AgNO3and 100 mg L-1 myoinositol, respectively. The embryogenic suspension cells formed somatic embryos at the frequency of 70 000~140 000 g-1 on the solid MS medium amended with 2% sucrose, 0.7% agar, 400 mg L- 1LH and 100 mg L-1 myoinositol. The somatic embryos were converted into plantlets at the frequency of over 65% after maturation. Plate 1, Fig 1, Tab 3, Ref 6

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Last Update: 2002-10-28