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Cloning and function study of tomato SUMO E3 ligase SIZ1-like1 gene(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2015 03
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Cloning and function study of tomato SUMO E3 ligase SIZ1-like1 gene
ZHANG Junfang TANG Xiaofeng LI Yuxiang DENG Heng GAO Lanyang LIU Yongsheng
State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Sciences, Sichuan University, Chengdu 610064, China
tomato SIZ1-like1 gene clone drought tolerance subcellular localizationpositive regulation
Q78 : S641.203.4

Sumoylation is an important way to regulate post-translation of proteins in plant cells, including protein degradation and location, responses to environmental stresses and hormones. SIZ1 is a SUMO (small ubiquitin-related modifier) E3 ligase that plays a vital role in sumoylation. In order to illustrate the function of SIZ1 gene in tomato, this research cloned SIZ1 gene from tomato, constructed SIZ1-like1-PBI121 over-expression and SIZ1-like1-pSKint RNA interference fusion expression vectors, and transformed them into wild type tomato using Agrobacterium-mediated transformation to successfully obtain six positive transgenic plants. The Real-Time PCR experiment indicated that SIZ1-like1 expressed in all growth stages of tomato. SIZ1-like1-YFP fusion expression vector was constructed and transformed into tomato. The results of microscopic observation revealed that SIZ1-like1 was located in the nuclei. Drought stress indicated that transgenic plants with overexpressed SIZ1-like1 had stronger tolerance compared to the wild type, with proline content about threefold of the latter; however, the SIZ1-like1RNA interference plants had less tolerance. These results demonstrated the positive regulation of SIZ1 gene in tomato.


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Last Update: 2015-06-23