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Inhibition of K562 cancer cells by lactonic diacetyl-sophorolipid(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2015 02
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Inhibition of K562 cancer cells by lactonic diacetyl-sophorolipid
CHEN Jing LIU Ran LIU Yuewen LIU Xinli
1School of Life Science, Shandong University, Jinan 250100, China 2Shandong Provincial Key Lab of Microbial Engineering, College of Food and Bioengineering, Qilu University of Technology, Jinan 250353, China
lactonic diacetyl-sophorolipid anticancer K562 necrosis
Q936 : R979.1

Sophorolipids (SLs) are important extracellular biosurfactants produced by yeasts. Among the major forms of sophorolipids, lactonic diacetyl-sophorolipids can be used as drugs for their pharmaceutical activity. This study aimed to investigate the anticancer activity of sophorolipids to broaden the scope of application. The effects of lactonic diacetyl-sophorolipid on K562 cells were studied by MTT method, phase contrast microscopy, fluorescent staining and DNA gel electrophoresis. The results showed that K562 cell growth was inhibited, with IC50 value as 31.70 μg/mL and 29.80 μg/mL after sophorolipid treatment for 24 h and 48 h, respectively. Morphological changes of K562 cells were observed including cell swelling, cell content overflow and cell lysis. Nuclear shrinkage, cleavage and dissolution appeared. Diffuse bands of DNA by gel electrophoresis indicated random DNA breakage. These results demonstrated that sophorolipids can exert anticancer activity on K562 cells which may be caused by necrosis.


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Last Update: 2015-04-28